问题 共线性分析画圈图的时候出现报错Width (0) and height (0) cannot be

Reading control file error: For input string: "1000    //plot width and height (in pixels)"Exception in thread "main" java.lang.IllegalArgumentException: Width (0) and height (0) cannot be <= 0    at java.desktop/java.awt.image.DirectColorModel.createCompatibleWritableRaster(DirectColorMode...

用户 The soul can only walk alone

问题 老师好，我在利用在SNP设计dcasp标记时得到的primers.result.xls文件为空。

...ne nt can be changed to fit enzyme NlaIII,CviAII,FatI,132 change position and primer end postions are  298 [299] CAPS found with enzyme  ScrFI,StyD4I,66 CAPS found with enzyme  FspEI,530 One nt can be changed to fit enzyme MspJI,530 change position and primer end postions are  297 [298, 299...

文章 转录本组装软件StringTie的使用说明

...ipt-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown”  其中StringTie 在组装转录本的完整度，精度和速度方面都较以往的cufflinks 有很大的提升。 StringTie 使用说明:   stringtie <input.bam ..> [-G <guide_gff>] [-l &l...

文章 R语言数据整理——tidyr包

...dxl)> data <- read_excel('data.xlsx')> data# A tibble: 8 × 2   type   num  <chr> <dbl>1     a    752     b    723  <NA>    664     a    NA5     c    696     b    657     a    728     c    NA 从上面的数据中，我们可以...

文章 lm()多元回归分析结果解读

...‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1Residual standard error: 0.5099 on 34 degrees of freedomMultiple R-squared:  0.9383,    Adjusted R-squared:  0.9328 F-statistic: 172.3 on 3 and 34 DF,  p-value: < 2.2e-16 mySummary = summary(myModel) mySummary[1]或mySummar...

问题 单细胞测序数据FindAllMaker查找不同Cluster的Marker基因

...如下：Rscript $scripts/seurat_FindAllMarkers.r --rds $workdir/05.cell_type_ann/pbmc.added.celltype.rds \  -p FindAllMarkers --test.use  DESeq2   --logfc.threshold 0.25 。我执行后报错：Warning: When testing 14 versus all:         every gene contains at least one zero, cannot comp...

文章 做微生物研究必懂的OTU table相关知识

...格，后续的分析就很好展开了。 参考文献：[1] T. Magoc and S. Salzberg. FLASH: Fast length adjustment of short reads to improve genome assemblies. Bioinformatics 27:21 (2011), 2957-63.[2] Patrick D. Schloss,et al. Introducing mothur: Open-Source, Platform-Independent, Community-...

问题 在用gemma进行亲缘关系矩阵计算时，已经給fam文件添加表型但还是报错

命令：/data/soft/gemma/gemma-0.98.5-linux-static-AMD64 -bfile /wtmp2/user049/yushiyan/prune_/alleledphwemafmissing_LDfiltered.snps -gk 2 -p /wtmp2/user049/yushiyan/-P/pheno.txt -o /wtmp2/user049/yushiyan/kinship/result.kinship_n21 ERROR: Enforce failed for number of analyzed individuals equals...

文章 超大基因组，超长染色体，凤丹牡丹基因组怎一个难字了得！

...Nature Communications（IF=17.69）上发表了题为“Genomic basis of the giga-chromosomes and giga-genome of tree peony Paeonia ostii”的研究论文。该研究公布了凤丹牡丹的高质量参考基因组，解析了凤丹牡丹巨大基因组的形成与维持机制；结合448份材料...

问题 没有发现CA凭证等。 “could not read CA certificate “C:\Users\username\.docker\machine\machines\default\ca.pem”: open C:\Users\yqiu29.docker\machine\machines\default\ca.pem: The system cannot find the file specified.”

没有发现CA凭证等。 “could not read CA certificate “C:\Users\username\.docker\machine\machines\default\ca.pem”: open C:\Users\yqiu29.docker\machine\machines\default\ca.pem: The system cannot find the file specified.”

问题 老师您好！我把顺势作用元件的名称都改成了大写的，但是出现了如下报错Request-URI Too Long The requested URL's length exceeds the capacity limit for this server.

老师您好！我把顺势作用元件的名称都改成了大写的，但是出现了如下报错然后根据提示更改设置，但是“具体参考”链接一直打不开还了几台电脑都不行，希望老师指点

问题 tassel 报错 SortGenotypeFilePlugin to correctly order the file.

ERROR net.maizegenetics.plugindef.AbstractPlugin - Problem loading file: /work/my_pop_cucu/00.filter/clean.vcf.gz. Error: BuilderFromVCF: Ordering incorrect. VCF file must be ordered by position. Please first use SortGenotypeFilePlugin to correctly order the file.

文章 快速了解转录调控家族的新贵-circRNAs

...用这个好用的软件CIRI。 5 参考文献 Yuan Gao, Jinfeng Wang, and Fangqing Zhao.CIRI: an efficient and unbiased algorithm for de novo circular RNA identification.Genome Biol. 2015; 16(1): 4. Chen G, Cui J, Jin B al.Genome-Wide Identification of Circular RNAs in Arabidopsis thaliana.Front...

问题 error: the following arguments are required这是什么原因