找到约 15 条结果

文章 immune_compare_stat.r 免疫细胞表达差异比较

.....], --celltype celltype [celltype ...]                        which celltype to compare : "B cell" ...[required]  -m filepath, --metadata filepath                        input metadata file path[required]  -g group, --group group                        group na...

文章 绘制展示基因在样本中表达量与数量的柱状图

... location of the legend[none,left,right,bottom,top,or two-element numeric vector][optional,default top] -o outdir, --outdir outdir output file directory[optional,default cwd] -p prefix, --prefix prefix ...

问题 index.sh构建索引时，其他文件都正常，但是这个unknown_refGeneMrna.fa文件是空的

WARNING: Cannot identify sequence for PH02Gene38415.t1 (starting from hic_scaffold_16:44053792) NOTICE: Finished writting FASTA for 0 genomic regions to unknown_refGeneMrna.fa

问题 docker 安装

...安装，出现报错界面Installation failed: Containers Windows Feature is not available，我已按照参考资料https://www.omicsclass.com/article/1303的步骤新建了containers.cmd，并以管理员身份运行完成后重启了电脑，再次安装时依然出现这样的界面，应该怎...

问题 在做叶绿体组装时，出现了如下错误，该如何解决呢

...93.19 s      62      63 ##############################      64 For trouble-shooting, please      65 Firstly, check https://github.com/Kinggerm/GetOrganelle/wiki/FAQ      66 Secondly, check if there are open/closed issues related at https://githu      67 If your problem was st...

文章 Orthology 定义

...ologs) 最早由Walter M. Fitch于1970年定义。 "It is not suffcient, for example, when reconstructing a phylogeny from amino acid sequences that the proteins be homologous. [. . . ] there should be two subclasses of homology. Where the homology is the result of gene duplication so that both co...

问题 重测序

... ret: 32512, going to retry. sh: -c: line 0: unexpected EOF while looking for matching `"' sh: -c: line 1: syntax error: unexpected end of file sh: -O: command not found warning, cmd: sh: -c: line 1: syntax error: unexpected end of file warning, cmd:   echo "RUN CMD: gatk --java-options '-Xmx...

文章 使用Dockerfile制作生物信息分析基础镜像

...ts\ cmake zlib libpng libpng12 libtiff libjpeg boost \ wget zip which less tree \ && yum clean all \ && mkdir -p /biosoft/miniconda \ && wget https://mirrors.tuna.tsinghua.edu.cn/anaconda/miniconda/Miniconda3-latest-Linux-x86_64.sh -O /tmp/miniconda.sh \ ...

问题 基因家族生信

perl ../script/get_gene_position.pl ../Arabidopsis_thaliana.TAIR10.41.gff3 AT.gff 您好，我运行上述指令的时候，出现了下列的错误 Can't open perl script "../script/get_gene_position.pl": No such file or directory 请问是什么问题呀

文章 perl中的列表与数组

...var, "a string")(17, 26 << 2)(17, $var1 + $var2)($value, "The answer is $value") 二、数组--列表的存贮 列表存贮于数组变量中，与简单变量不同，数组变量以字符"@"打头，如： @array = (1, 2, 3); 注：  (1)数组变量创建时初始值为空列表：(...

问题 MCSCANX共线性图代码报错

在使用MCSCANX进行绘图时检查了路径和目录 但是一直提示 Could not find or load main class family_circle_plotter 特来请教各位该如何解决

问题 TCGA下载数据保存

下载FPKM的时候，保存结果时出现Error in read_connection_(con) : Evaluation error: error reading from the connection. In addition: Warning message: In (function (con, what, n = 1L, size = NA_integer_, signed = TRUE,  :   invalid or incomplete compressed data

问题 使用SummarizedExperiment包的assay提取miRNA数据的表达矩阵时报错是什么原因？

...ction (classes, fdef, mtable)  :    unable to find an inherited method for function ‘assay’ for signature ‘"data.frame", "missing"’

问题 IBD计算1

IBD 计算时有一步错误为cat: chr*.ibd: No such file or directory,  我的染色体是1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29。  这一步怎么该为数字为前缀的文件的ibd 合并到一块，原始命令是cat chr*.ibd > sativa.ibd

文章 鉴定组装植物病毒——系统详尽的植物病毒学！

.... . . . . . ................. . . . 12 RT-PCR and Real-Time RT-PCR Methods for the Detectionof Potato Virus Y in Potato Leaves and Tubers. . . ......................................................................  133 A New Method to Isolate Total dsRNA. . . . . . . ..................................