找到约 15 条结果

问题 WGCNA分析中,导入txt的性状数据时(数据为12行样本,10列性状数据),报错Error in scan(file = file, what = what, sep = sep, quote = quote, dec = dec, : line 1 didot have 12 elements。但是数据如果是12行样本,8列性状,不会报错

...xt",row.names=1,header=T,comment.char = "",check.names=F)但是报错Error in scan(file = file, what = what, sep = sep, quote = quote, dec = dec,  :    line 1 did not have 12 elements。但是数据如果是12行样本,8列性状,就不会报错。谢谢

问题 在运行这段代码时出现Invalid value for '--m-metadata-file': Metadata column is of type 'numeric', but expected Categorical.

...自metadata文件  #--p-mode <up to you, there's sum, median-ceiling, and mean-ceiling>   qiime feature-table group \      --i-table feature-table-final.qza \      --p-axis sample \      --m-metadata-file $metadata \      --m-metadata-column $g \      --p-mode sum  \ ...

文章 IF=4.8 | 联合转录组与代谢组分析揭示了硒对百合生长和品质的影响

...tiers in plant science杂志发表了一篇题为“Combined transcriptome and metabolome analyses reveal the effects of selenium on the growth and quality of Lilium lancifolium”的转录组代谢组联合分析文章。” 01 — 研究背景 卷丹百合(学名:Lilium lancifolium Thunb...

文章 正选择分析之 Site Models

...作用的检测,而是用于位点间ω值是否一致的检测。 M1 and M2 以及M7 and M8是用于正选择作用的检测,作者推荐使用这两组比较进行LRT检验来验证正选择。不过Prof.Yang认为,The M1-M2 comparison 与 the M7- M8 comparison相比,更加的稳定。M7...

问题 请问QQ图绘制时报错,文件明明在,却找不到文件怎么回事啊?

> library(qqman) > results_log = read.table("logistic_results.assoc.logistic", head=TRUE) Error in file(file, "rt") : cannot open the connection In addition: Warning message: In file(file, "rt") :   cannot open file 'logistic_results.assoc.logistic': No such file or directory

问题 检查数据是否正确

...=1,header=T,comment.char = "",check.names=F) > allowWGCNAThreads() Allowing multi-threading with up to 8 threads. > ALLOW_WGCNA_THREADS=4 > memory.limit(size = 20000) [1] 32457 Warning message: In memory.limit(size = 20000) : cannot decrease memory limit: ignored > # 查看部分样本...

问题 vcftools --gzvcf all.varFilter.vcf.gz --recode --recode-IN.............els |gzip - > clean.vcf.gz这一步输出的vcf.gz文件,用 bcftools query -l clean.vcf.gz | wc -l查看样本名出现报错

Failed to read from clean.vcf.gz: unknown file type 0 这是为什么呢

文章 最新基因家族分析文献详解-冬枣扩展蛋白基因家族分析-11月8日接收

...:2区)上的题为: Genome‑wide identifcation, characterization, and expression analysis of the expansin gene family in Chinese jujube (Ziziphus jujuba Mill.) 的冬枣中扩展蛋白家族(expansin gene family)分析文章,一起来看一看,基因家族需要做哪些分析就能...

问题 perl求助,基因ID的对应替换perl脚本怎么写

...的Bna基因ID对应替换上去,perl脚本该怎么写。。 #!/usr/bin/perl ;#usage:perl repalce.pl list.file fa.file out.fileopen LIST,$ARGV[0];open FAFILE,$ARGV[1];open OUT,">$ARGV[2]";undef $/;#$in=<FAFILE>;#study $in;$/="\n";#while(<LIST>){    chomp;    my($new,$old)=...

问题 perl求助,基因ID的对应替换perl脚本怎么写

...的Bna基因ID对应替换上去,perl脚本该怎么写。。 #!/usr/bin/perl ;#usage:perl repalce.pl list.file fa.file out.fileopen LIST,$ARGV[0];open FAFILE,$ARGV[1];open OUT,">$ARGV[2]";undef $/;#$in=<FAFILE>;#study $in;$/="\n";#while(<LIST>){    chomp;    my($new,$old)=...

文章 转录组测序分析数据库介绍

...16个主要的数据库,并用不同的颜色进行区分。 Genetic Information Processing (遗传信息处理)Metabolism (新陈代谢) Environmental Information Processing (环境信息处理) Cellular Processes (细胞过程) Organismal Systems (生物系统) ...

文章 BWA比对及Samtools提取mapped reads

...: 1:Only READ1 is set. 2:Only READ2 is set. O:Either both READ1 and READ2 are set;or neither is set. 对于PE测序reads,同时指定-1 R1.fq-2 R2.fq-s singleton.fq 时,samtools会将flag1和flag2配对的序列分别输出到-1-2指定的文件,对于无法匹配的flag 1/2,...

文章 biolinux安装matplotlib时报错

biolinux的Ubuntu系统上安装matplotlib时报错: sudo pip install matplotlib 遇到报错: Downloading tornado-5.0.2.tar.gz (506kB): 506kB downloaded   Running setup.py (path:/tmp/pip_build_root/tornado/setup.py) egg_info for package tornado     Traceback (most recent call last): ...

文章 计算Kaks时批量提取多对基因的序列

...gt;" unless ( @ARGV == 3 );use Math::BigFloat;use Bio::SeqIO;use Bio::Seq;$in = Bio::SeqIO->new(        -file   => "$ARGV[1]",        -format => 'Fasta');my %gene;while ( my $seq = $in->next_seq() ) {        my ( $id, $sequence, $desc ) = ( $seq->id, $seq->se...

文章 ValueError: invalid literal for int() with base 10: ''

...绘制共线性绘图时报错如下: 运行命令如下: /biosoft/miniconda/miniconda2/bin/python -m jcvi.graphics.karyotype  --format=pdf  --figsize=15x5 mcscan_seqid mcscan_layout 报错信息如下: markers', 'networkx.algorithms.community.__future__', 'networkx.generators.atlas', 'n...