问题 老师您好! 我在用samtools获取染色体长度时有一个different line length in sequence contig1报错，请问怎么处理啊

问题 infercnv error in readrds(reload_info$expected_file_names[[i]]) : error reading from connection calls: -> readrds execution halted

infercnv error in readrds(reload_info$expected_file_names[[i]]) : error reading from connection calls: <anonymous> -> readrds execution halted

问题 问一下老师们，在用get_genefamily_bed.pl 这个脚本，提取自己基因家族位置信息的结果文件总是为空，下面是为的输入文件。perl get_genefamily_bed.pl -in1 SAT.IDlist.txt -in2 Oryza_sativa.IRGSP-1.0.47.gff3 -out MtN3_SAT.bed执行的是条命令。

问题 meme分析报错：Illegal character `U' in sequence LOC105388627. Change alphabet or fix data file.

请问老师，是根据最终得基因ID提取得蛋白质序列，为什么在做meme分析时，会出现这种错误？

问题 Error in (function (classes, fdef, mtable) : unable to find an inherited method for function ‘bg.correct’ for signature ‘"HTAFeatureSet", "character"’

对GEO数据进行mas5处理时报错。。不知道如何处理？？ Error in (function (classes, fdef, mtable)  :    unable to find an inherited method for function ‘bg.correct’ for signature ‘"HTAFeatureSet", "character"’

问题 如何修改perl脚本从cds文件中提取上游序列呢？（有些cds中的scaffold可能不足1500）

... unless(@ARGV==3 ); use Math::BigFloat; use Bio::SeqIO; use Bio::Seq; $in = Bio::SeqIO -> new(-file => "$ARGV[0]",                                   -format => 'Fasta'); $out = Bio::SeqIO -> new(-file => ">$ARGV[2]",                            ...

问题 抱歉打扰，我在做circos时遇到了下面问题，看到了您也遇到相似问题，想知道您是如何解决的，期待您的回复。

...anager/share/laccasegenefamily/laccasecircos debuggroup summary 0.43s command /biosoft/circos/circos-0.69-6/bin/circos -conf /home/manager/share/laccasefamily/laccasecircos/config3.txt -outputdir ./ -outputfile ABRE3a debuggroup summary 0.43s loading configuration from file /home/manager/share/lac...

问题 有参转录组分析第五步5.deg Error in newCountDataSet报错

...老师帮忙解答一下，万分感谢。  [ reached getOption("max.print") -- omitted 4 rows ] [1] "create DEG_list object is over" [1] "the number of origin gene is 27781" [1] "the number of gene (after filtering) is 17256" Error in newCountDataSet(countData = de$filterCounts, conditions = ...

文章 从MEGA进化树分析结果nwk文件提取基因ID

...一脚本，可以实现此操作。 用法： perl nwk_geneid.pl  -i in.nwk  -o out.txt in.nwk 为输入的nwk文件，out.txt是输出的基因ID文件。 脚本代码； use Getopt::Long;use strict;my %opts;GetOptions(\%opts,"i=s","o=s","h");open(IN,"$opts{i}") || die "open $opts{i} faile...

问题 Linux服务器上，运行提取结构域的脚本的时候，出现未安装Bio/SeqIO.pm in @INC的错误，请问如何解决

这样的在服务器上在安装biolinux吗

文章 igraph 报错： libglpk.so.36: cannot open shared object file: No such file or directory

** demo ** inst ** byte-compile and prepare package for lazy loading ^A** help *** installing help indices *** copying figures ** building package indices ** installing vignettes ** testing if installed package can be loaded from temporary location Error: package or namespace load failed fo...

问题 请问老师，使用annovar进行注释出现报错，如下：Error: invalid record found in exonic_variant_function file (exonic format error)

...，这一次添加完报错信息是：在输出文件时无法识别格式invalid record found in annovar outputfile:。是不是软件没有更新？annovar/2019Oct24/annotate_variation.pl 我用的是这一版。还是说有一些低质量没有过滤的snp导致了识别错误，我上一步的...

问题 Blast2go本地化过程中，运行官方文件b2g4pipe_v2.5.zip中“runPipeExample.sh“报错“The host did not accept the connection within timeout of 60000 ms;”

...ter (thin-out) graph ... Number of nodes: 62 for molecular_function Graph construction finished ... Save Graph Calculate graph layout Generating DOT file Generating DOT file Graph Layout: GONAMEANDID Graph Layout: GONAMEANDID false Graph-Layout-Webservice has problems or is not avalible from: http:...

问题 R语言GAPIT跑GWAS错误 Error in ‘[.data.frame‘(GD,,!is.na(GI.MP[,3])):选择了未定义的列

问题 MCscan运行问题：ZeroDivisionError: float division by zero

... Traceback (most recent call last):  File "/usr/lib/python2.7/runpy.py", line 174, in _run_module_as_main    "__main__", fname, loader, pkg_name)  File "/usr/lib/python2.7/runpy.py", line 72, in _run_code    exec code in run_globals  File "/usr/local/lib/python2.7/dist-packages/jcvi/graphic...