问题 Perl GD模块官网例子报错

...e = $im->colorAllocate(0,0,255);   # make the background transparent and interlaced $im->transparent($white); $im->interlaced('true');   # Put a black frame around the picture $im->rectangle(0,0,99,99,$black);   # Draw a blue oval $im->arc(50,50,95,75,0,360,$blue);   #...

问题 老师您好！lumi包读取文件出错。Error in gregexpr("\t", dataLine1)[[1]] : 下标出界

...的时候，自己的数据读取不出（格式不对）。今天读illumina的文件，又读取不出。 课堂提供的代码和例子是没有问题的。下面分别是报错、例子的表头、自己的数据的表头。该如何解决？

问题 blast2go报错，升级blast ncbi-blast-2.3.0+ 到 ncbi-blast-2.6.0+ 报错

Can not parse BLAST XML because can not find information about the used version!

文章 Perl方法对文档全文进行字符串对应替换

... getcwd); my %opts; GetOptions (\%opts,"idlist=s","gff=s","out=s"); if(!defined($opts{idlist})||!defined($opts{gff})||!defined($opts{out})){ &USAGE; } ######################get gff############################## my $gff; open(IN,"$opts{gff}")||die "open gff failed\n"; { local $/=unde...

问题 fastqc质控时报错，提示Ran out of data in the middle of a fastq entry.是什么问题？

文章 perl语言标量SCALAR

...度：指数范围：-309~308 太小的话显示成0，太大显示为1.#INF   4.数字操作 加减乘除  + -  *  / 二、字符串 perl中的字符串必须必须用引号引起来，可以是单引号，也可以是双引号； 以下引起来的都是字符串： "omicsclass""daf...

文章 qiime2 couldn't connect to display "localhost:12.0"

Traceback (most recent call last): File "/share/work/biosoft/conda/Miniconda3.8/envs/qiime2-2020.8/lib/python3.6/site-packages/q2cli/commands.py", line 329, in __call__ results = action(**arguments) File "<decorator-gen-545>", line 2, in summarize File "/share/work/biosoft/conda/Mini...

问题 Python 版mcscanx出现AttributeError: 'LayoutLine' object has no attribute 'order'

... file `at.bed` Traceback (most recent call last):   File "/home/chenxh/miniconda3/envs/jcvi/lib/python2.7/runpy.py", line 174, in _run_module_as_main     "__main__", fname, loader, pkg_name)   File "/home/chenxh/miniconda3/envs/jcvi/lib/python2.7/runpy.py", line 72, in _run_code     exec ...

问题 http://mg2c.iask.in/mg2c_v2.0/ 总是打不开

老师，这个网站打不开，是需要翻墙么？ 还是网站歇菜呢？ 在染色体位置和转录本结构分析时有没有其他可以代替的网站或者软件。

问题 老师您好! 我在用samtools获取染色体长度时有一个different line length in sequence contig1报错，请问怎么处理啊

问题 infercnv error in readrds(reload_info$expected_file_names[[i]]) : error reading from connection calls: -> readrds execution halted

infercnv error in readrds(reload_info$expected_file_names[[i]]) : error reading from connection calls: <anonymous> -> readrds execution halted

问题 问一下老师们，在用get_genefamily_bed.pl 这个脚本，提取自己基因家族位置信息的结果文件总是为空，下面是为的输入文件。perl get_genefamily_bed.pl -in1 SAT.IDlist.txt -in2 Oryza_sativa.IRGSP-1.0.47.gff3 -out MtN3_SAT.bed执行的是条命令。

问题 Blast2go本地化过程中，运行官方文件b2g4pipe_v2.5.zip中“runPipeExample.sh“报错“The host did not accept the connection within timeout of 60000 ms;”

...ter (thin-out) graph ... Number of nodes: 62 for molecular_function Graph construction finished ... Save Graph Calculate graph layout Generating DOT file Generating DOT file Graph Layout: GONAMEANDID Graph Layout: GONAMEANDID false Graph-Layout-Webservice has problems or is not avalible from: http:...

问题 抱歉打扰，我在做circos时遇到了下面问题，看到了您也遇到相似问题，想知道您是如何解决的，期待您的回复。

...anager/share/laccasegenefamily/laccasecircos debuggroup summary 0.43s command /biosoft/circos/circos-0.69-6/bin/circos -conf /home/manager/share/laccasefamily/laccasecircos/config3.txt -outputdir ./ -outputfile ABRE3a debuggroup summary 0.43s loading configuration from file /home/manager/share/lac...

问题 Error in (function (classes, fdef, mtable) : unable to find an inherited method for function ‘bg.correct’ for signature ‘"HTAFeatureSet", "character"’

对GEO数据进行mas5处理时报错。。不知道如何处理？？ Error in (function (classes, fdef, mtable)  :    unable to find an inherited method for function ‘bg.correct’ for signature ‘"HTAFeatureSet", "character"’