文章 TukeyHSD报错

Error in TukeyHSD.aov(., ...) : no factors in the fitted model Calls: %>% ... is.data.frame -> <Anonymous> -> TukeyHSD -> TukeyHSD.aov In addition: Warning message: In replications(paste("~", xx), data = mf) : non-factors ignored: group1 Execution halted 数据框组代表...

问题 报错出现“Non-unique name 'IRIS_313-7' in the alignment”

请教一下，我在构建进化树的时候出现这个报错，是不是因为我整理数据的时候有重复的单个文件呀？遇到这种情况，我应该怎么处理呢？

问题 MCsanX运行报错ZeroDivisionError: float division by zero

Traceback (most recent call last):   File "/biosoft/miniconda/miniconda2/lib/python2.7/runpy.py", line 174, in _run_module_as_main     "__main__", fname, loader, pkg_name)   File "/biosoft/miniconda/miniconda2/lib/python2.7/runpy.py", line 72, in _run_code     exec code in run_globals   ...

问题 metastat Error in `[.data.frame`(df, , c("#OTU ID", groupA, groupB)) : undefined columns selected Calls: [ -> [.data.frame

Error in `[.data.frame`(df, , c("#OTU ID", groupA, groupB)) :   undefined columns selected Calls: [ -> [.data.frame

问题 qiime2 python "matplotlib" _tkinter.TclError: couldn't connect to display "localhost:12.0" 报错

Traceback (most recent call last):  File "/share/work/biosoft/conda/Miniconda3.8/envs/qiime2-2020.8/lib/python3.6/site-packages/q2cli/commands.py", line 329, in __call__    results = action(**arguments)  File "<decorator-gen-404>", line 2, in summarize  File "/share/work/biosoft/conda/Min...

文章 docker clash 配置

...0.1:7890" Environment="HTTPS_PROXY=http://127.0.0.1:7890" Flush changes and restart Docker$ sudo systemctl daemon-reload $ sudo systemctl restart docker

问题 老师，这是在组学大讲堂找的三个物种做比较基因组学分析的一个脚本，运行显示Can't find string terminator " Usage End." anywhere before EOF at ../script/mcscan.filter.pl line 13.的错误，请老师看下这个脚本，谢谢老师

...name dirname);use Cwd qw(abs_path);#get optsmy %opts;GetOptions(\%opts, "in=s", "out=s","id1=s","id2=s","h");if(!defined($opts{id2}) || !defined($opts{id1}) || !defined($opts{in}) || !defined($opts{out}) || defined($opts{h})){ print <<" Usage End."; Usage Forced pa...

问题 getGEO函数不能下载，提示Error in open.connection(x, "rb") : cannot open the connection，谢谢！

问题 基因组重测序，变异结果注释的时候出现了Error: invalid record found in annovar outputfile

输入的命令，出现的错误，产出的文件如上。感谢各位大佬。

问题 构建进化树报错

构建进化树时报错：Oops! MEGA has encountered an error and cannot continue with this analysis:Some pairwise distances could not be estimated. For example, between sequences 25 and 12.这是为什么啊？

文章 qiime2自己建立分类器 greengene

... 这里以V3-V4区引物为例： 338F (5′-ACTCCTACGGGAGGCAGCAG-3′) and. 806R (5′-GGACTACHVGGGTWTCTAAT-3′) # 下载数据库文件(greengenes) wget -c ftp://greengenes.microbio.me/greengenes_release/gg_13_5/gg_13_8_otus.tar.gz # 解压 tar -zxvf gg_13_8_otus.tar.gz # 使用rep...

问题 Linux服务器上，运行提取结构域的脚本的时候，出现未安装Bio/SeqIO.pm in @INC的错误，请问如何解决

Linux服务器上，运行提取结构域的脚本的时候，出现未安装Bio/SeqIO.pm in @INC的错误，请问如何解决，这个问题之前有提过，但是没有人回答，有人只是指出可以安装docker，但是需要root用户安装，没有root权限的用户怎么办

文章 CentOS 7 yum安装报错

...org; Unknown error" One of the configured repositories failed (Unknown), and yum doesn't have enough cached data to continue. At this point the only safe thing yum can do is fail. There are a few ways to work "fix" this:     1. Contact the upstream for the repository and get them to fix the pr...

问题 问一下老师们，提取成对基因的时候得到结果文件总是为空是什么原因，下面是我的输入文件perl ../script/KAKS_SHAIXUAN.pl -in1 WRKY_cds_confirmed.fa.fai -in2 WRKY_cds_confirmed_blast.out -out duplication_gene.out就是这条命令。

问题 pip is configured with locations that require TLS/SSL, however the ssl module in Python is not available

python 编译之后报错： pip is configured with locations that require TLS/SSL, however the ssl module in Python is not available