找到约 15 条结果

问题 使用GATK的GenomicsDBImport合并gvcf导入db时报错Unexpected compressed block length: 1 for /work/GATK/BN14.g.vcf.gz

...---------------------------------- 10:53:02.159 INFO  GenomicsDBImport - The Genome Analysis Toolkit (GATK) v4.4.0.0 10:53:02.160 INFO  GenomicsDBImport - For support and documentation go to https://software.broadinstitute.org/gatk/ 10:53:02.160 INFO  GenomicsDBImport - Executing as root@1fea4...

文章 群体结构分析structure原理

...ence, 2009, 326(5951): 433-436.[3]Evanno G, Regnaut S, Goudet J. Detecting thenumber of clusters of individuals using the software STRUCTURE: a simulationstudy[J]. Molecular ecology, 2005, 14(8): 2611-2620.[4] Porras-Hurtado L, Ruiz Y, Santos C, et al. An overview of STRUCTURE:applications, paramet...

问题 利用KaKs_Calculator2.0计算两个基因的kaks时出现:Error.

The size of two sequences in 'EVM0055308&EVM0007023' is not equal. 出现问题后也按照课堂上和视频课程中的方法进行了解决,也检查了ATG,序列是否为3的倍数等问题,均符合要求,不知道是什么问题造成的,

问题 关于使用nohup vcftools过滤vcf文件的问题

...t;##FORMAT=<ID=AD,Number=R,Type=Integer,Description="Allelic depths for the ref and alt alleles in the order listed">##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Approximate read depth (reads with MQ=255 or with bad mates are filtered)">##FORMAT=<ID=GQ,Number=1,Type=Integer,Desc...

问题 群体结构分析时,执行代码报错

...vironment value of R_HOME --> Q&A for bioinformatics, please visit the website: https://www.omicsclass.com/ --> R beginners ? I suggest your  learning  R language: https://study.omicsclass.com/index pophelper v2.3.1 ready. Error in plotQ(qlist, imgoutput = "join", returnplot = T, exp...

问题 Bioconductor包安装时出现警告,导致最后出来的图片部分缺失

...Lite for help A new version of Bioconductor is available after installing the most recent   version of R; see http://bioconductor.org/install > biocLite(c("genefilter","ballgown","edgeR")) BioC_mirror: https://bioconductor.org Using Bioconductor 3.2 (BiocInstaller 1.20.3), R 3.2.3 (2015-12-...

文章 蛋白质的理化性质除了用Expasy网站还能用TBtools等分析

... 相对分子质量 (KDa)、等电点(PI)、负电荷残基(Total number of negatively charged residues)、正电荷残基(Total number of positively charged residues)、不稳定系数(Instability index)、总平均亲水性(Grand average of hydropathicity-简写GRAVY)、脂肪指数(Alipha...

问题 gatk call SNP样品多采取方法2,报错如下是怎么回事啊

A USER ERROR has occurred: Argument emit-ref-confidence has a bad value: Can only be used in single sample mode currently. Use the --sample-name argument to run on a single sample out of a multi-sample BAM file

问题 基因家族分析时用大豆gff文件报错 killed

...想要进行“保留蛋白编码基因”出现报错“=> Version of the Bioperl GFF parser selected by AGAT: 3 gff3 reader error level1: No ID attribute found @ for the feature: 1    ena_assembly_gap        biological_region      2584646                               ...

问题 Error in seq.default(grps[start] + 1, grps[start + 1] - 1) : 'from' must be of length 1此外: Warning messages: 1: In file(con, "r") : InternetOpenUrl失败:’不能连接到吊销服务器,或者未能获得最终响应。' 2: In readLines(...) : 读'http://bioconductor.org/config.yaml'时最后一行未遂

在rstudio中运行 BiocManager::install("TCGAbiolinks"),迟迟无法下载数据,并显示上方错误,请问怎么解决

文章 R语言包安装方法,设置国内镜像加快安装速度

.../TUNA Team, Tsinghua Universityhttps://mirrors.ustc.edu.cn/CRAN/University of Science and Technology of Chinahttp://mirrors.ustc.edu.cn/CRAN/University of Science and Technology of Chinahttps://mirror.lzu.edu.cn/CRAN/Lanzhou University Open Source Societyhttp://mirror.lzu.edu.cn/CRAN/Lanzhou Univers...

问题 单倍型分析中最后一步失败,麻烦老师

...vironment value of R_HOME --> Q&A for bioinformatics, please visit the website: https://www.omicsclass.com/ --> R beginners ? I suggest your  learning  R language: https://study.omicsclass.com/index Error in library(geneHapR) : there is no package called ‘geneHapR’ Execution halt...

问题 进行16S分析时,对样品按照分组进行分类汇总出现问题。

...pecies,data,Des AWGB16SAA21341083628R,MCon,MCon_1,F36/R28,NA,NA,House fly,2021/10/1,16s AWGB16SAA21341203728R,MCon,MCon_2,F37/R28,NA,NA,House fly,2021/10/1,16s AWGB16SAA21341323828R,MCon,MCon_3,F38/R28,NA,NA,House fly,2021/10/1,16s AWGB16SAA21341453929R,MCon,MCon_4,F39/R29,NA,NA,House fly,2021/1...

问题 hmmersearch出现错误Error: Failed to open sequence file globins45 for reading,或者globins45换成globins45.fa也会出现同样的提示,这是什么原因呢,需要怎样操作才能正常运行呢?谢谢!

文章 deseq_analysis.r 差异基因分析DESeq2

...] [optional, default: 1] -X x.lab, --x.lab x.lab the label for x axis [optional, default: log2FC] -Y y.lab, --y.lab y.lab the label for y axis [optional, default: -log10(FDR)] -T title, --title title the label for main tit...