找到约 15 条结果

问题 Perl GD模块官网例子报错

...locate(255,0,0);       $blue = $im->colorAllocate(0,0,255);   # make the background transparent and interlaced $im->transparent($white); $im->interlaced('true');   # Put a black frame around the picture $im->rectangle(0,0,99,99,$black);   # Draw a blue oval $im->arc(...

问题 求助大佬,circos图chr.info文件怎么整理?

circos报错:You did not define a karyotype file. Are you sure your configuration file is well formed? chr.info文件如图所示,全在Linux环境下用脚本整理的,是我哪里搞错了?还是数目太多?(用的scaffold,MCScanX也是,全部600个scaffold放到配置文件就...

问题 tophat2建立索引报错:Error: gtf_to_fasta returned an error.

...v2.1.1-----------------------------------------------[2019-04-28 05:49:42] Checking for Bowtie                  Bowtie version:        2.2.7.0[2019-04-28 05:49:44] Checking for Bowtie index files (genome)..        Found both Bowtie1 and Bowtie2 indexes.[2019-04-28 05:49:44] Checking...

文章 好用的perl模块推荐

...录要创建,可以使用以下的代码 use Path::Tiny;path("path/to/your")->mkpath; 删除也同样有很多功能可以高效化你的脚本而不需要每次都判断 # 删除空目录或文件,如果目录不为空,这个方法将会失败。path("path/to/your/file.txt")->remov...

问题 请问mapman分析kegg时,总提示这个 Data does not fit mapping used (no data points mapped),please choose the appropriate mapping which matces your data

请问mapman分析kegg时,总提示这个 Data does not fit mapping used (no data points mapped),please choose the appropriate mapping which matces your data

问题 ALLHIC1挂载出现问题

... 7       8 Find all occurrences of a motif in a genome.  Make a 'POS' file listing these occurrences, and also a BED file re       9      10 SYNTAX: make_bed_around_RE_site.pl <fasta> <motif> <range>      11 fasta:  A fasta file representing a genome (reference or...

问题 tassel 报错 SortGenotypeFilePlugin to correctly order the file.

ERROR net.maizegenetics.plugindef.AbstractPlugin - Problem loading file: /work/my_pop_cucu/00.filter/clean.vcf.gz. Error: BuilderFromVCF: Ordering incorrect. VCF file must be ordered by position. Please first use SortGenotypeFilePlugin to correctly order the file.

问题 16S Picrust2

...出现了下面的问题 问题: --> Q&A for bioinformatics, please visit the website: https://www.omicsclass.com/ --> R beginners ? I suggest your  learning  R language: https://study.omicsclass.com/index Failed to create bus connection: No such file or directory ── Attachi...

文章 外源插入流程报错运行不了

...ages/aimhii/__init__.py", line 153, in run_fastqjoin    subprocess.check_output(cmd, preexec_fn=lambda: signal.signal(signal.SIGPIPE, signal.SIG_DFL))  File "/share/work/biosoft/python/Python-v2.7.11/lib/python2.7/subprocess.py", line 566, in check_output    process = Popen(s...

文章 gdc-client 下载TCGA数据 报错 RROR: {"message":"Your token is invalid or expired. Please get a new token from GDC Data Portal."}

需要token  使用说明:https://docs.gdc.cancer.gov/Data_Transfer_Tool/PDF/Data_Transfer_Tool_UG.pdf

问题 使用plink做pca分析时报错

...de 'chr27' on line 30616892 of .vcf file.(This is disallowed for humans.  Check if the problem is with your data, or ifyou forgot to define a different chromosome set with e.g. --chr-set.) 使用的命令如下: plink --vcf  $workdir/2022_clean.vcf.gz --pca 10 --out  plink_pca   \    --a...

问题 16S 差异比较使用compare_errorbar.r的时候报错

...SD_Ligatur_vsKD_Ligatur_ppiL6 > done --> Q&A for bioinformatics, please visit the website: https://www.omicsclass.com/ --> R beginners ? I suggest your learning R language: https://study.omicsclass.com/index Failed to create bus connection: No such file or directory ── Attaching c...

问题 在基因家族分析过程中,进化树的构建过程中遇到了如下图的问题:完全按照视频要求删减后加载,并经行的分析。insufficient data available for ml analysis.please validate your sequence data

问题 利用cicro绘图时遇到错误

...       http://groups.google.com/group/circos-data-visualization   Please include this error, all your configuration, data files and the version   of Circos you're running (circos -v). Do not email me directly -- please use   the group.   Stack trace:  at /share/work/biosoft/circos...

问题 运行go_enrich.r报错

...: ignoring environment value of R_HOME --> Q&A for bioinformatics, please visit the website: https://www.omicsclass.com/ --> R beginners ? I suggest your  learning  R language: https://study.omicsclass.com/index Failed to create bus connection: No such file or directory Error: packag...