找到约 15 条结果

问题 老师，请问sample type出现这种情况怎么解决，麻烦老师解惑一下，谢谢啦。

...Methylation"> platform <- "Illumina Human Methylation 27"> sample_type <- "Primary solid Tumor" > legacy <- FALSE> # 设置工作目录（输出目录）> setwd(work_dir)> DataDirectory <- paste0(work_dir,"/GDC/",gsub("-","_",tcga_project))> FileNameData <- paste...

文章 GWAS分析哪个模型最优

...并绘制QQ图。CW trait (H = 80% 下图A) 关联结果看, ANOVA, GLM, and SUPER假阳性高；MLM, CMLM, and ECMLM这几个模型只是尾部少量SNP翘起说明假阳性减少但可能存在假阴性，因为尾部翘起的点较少。而FarmCPU 尾部高高翘起而且多说明这个模型假...

文章 plink 软件core dumped报错

...variants loaded from .bim file.9696 missing IDs set.114 people (0 males, 0 females, 114 ambiguous) loaded from .fam.Ambiguous sex IDs written to LDfiltered.nosex .--extract: 435 variants remaining.Using 1 thread (no multithreaded calculations invoked.Before main variant filters, 114 founders and 0 n...

问题 16S 差异比较使用compare_errorbar.r的时候报错

...trieve the full column specification for this data. ℹ Specify the column types or set `show_col_types = FALSE` to quiet this message. Error in `select()`: ! Can't subset columns with `metaData[, 1, drop = T]`. ✖ Can't convert from `metaData[, 1, drop = T]` <double> to <integer> due t...

问题 blastp提示ensemble中提取的序列文件不对

...ameters due to an invalid query sequence or its translation. Please verify the query sequence(s) and/or filtering options 已去除序列中的终止子符号。 e.g： >ENST00000620518 ENSG00000081692  JMJD4 1:227732392-227735273(-) MDRETRALADSHFRGLGVDVPGVGQAPGRVAFVSEPGAFSYADFVRGFLLPNLPCVF...

文章 基因组组装GC 含量与深度图：

...lab.org/seurat/reference/dimplot')parser$add_argument( "-i", "--gc.table", type="character",required=T, default=NULL,help="input single Seurat obj in rds file [required]",metavar="filepath")parser$add_argument( "-d", "--depth.table", type="character",required=T, default=NULL,help="input single Seura...

问题 成功安装WGCNA包后，调用时报错

....tuna.tsinghua.edu.cn/CRAN/bin/windows/contrib/3.5/WGCNA_1.66.zip' Content type 'application/zip' length 3436551 bytes (3.3 MB) downloaded 3.3 MBpackage ‘WGCNA’ successfully unpacked and MD5 sums checkedThe downloaded binary packages are in C:\Users\NY\AppData\Local\Temp\RtmpYrZpG5\downloaded_p...

文章 16S扩增子测序后的PICRUSt预测功能分析原理

...便快捷，成本也更低。 2016年发表在微生态学旗舰期刊《TheISME Journal》的《Cigarettesmoking and the oral microbiome in a large study of American adults》[2]中，作者就通过PICRUSt预测了吸烟人群和未吸烟人群的口腔菌群，发现共有83个基因功能代...

文章 R脚本报错|URL 'https://raw.githubusercontent.com/YinLiLin/R-CMplot/master/R/CMplot.r': Timeout of 60 seconds was reached

...时。 Error in file(filename, "r", encoding = encoding) :  cannot open the connection to 'https://raw.githubusercontent.com/YinLiLin/R-CMplot/master/R/CMplot.r'Calls: source -> fileIn addition: Warning message:In file(filename, "r", encoding = encoding) :  URL 'https://raw.githubusercontent.c...

问题 重测序中两个GATK运行都失败，显示如下代码和图片，麻烦老师进行解答

...oadinstitute/hellbender/Main has been compiled by a more recent version of the Java Runtime (class file version 61.0), this version of the Java Runtime only recognizes class file versions up to 55.0

问题 braker报错

... 16 17:02:31 2024: Did not find environment variable $AUGUSTUS_BIN_PATH (either variable does not exist, or the path given in variable does not exist). Will try to set this variable in a different way, later. # Tue Jan 16 17:02:31 2024: Trying to guess $AUGUSTUS_BIN_PATH from $AUGUSTUS_CONFIG_PATH....

文章 基因家族进化分析 发10分文章

..., Zhou, Y. and Jiao, Y. (2020), Evolutionary strategies drive a balance of the interacting gene products for the CBL and CIPK gene families. New Phytol, 226: 1506-1516. https://doi.org/10.1111/nph.164453. Yong-Chao Xu, Xiao-Min Niu, Xin-Xin Li, Wenrong He, Jia-Fu Chen, Yu-Pan Zou, Qiong Wu, Yon...

问题 vegan包问题请教

...m")   > png(file="spe.png",h=2000,w=2000,res=300) > plot(all, ci.type = "poly", col = "blue", lwd = 2, ci.lty = 0, +      ci.col = "lightblue", main = "Species Accumulation Curves", xlab = "Number of samples", +      ylab = "Number of  species") > boxplot(all, col = "yellow", ...

问题 vegan包问题请教

...andom")  > png(file="spe.png",h=2000,w=2000,res=300)> plot(all, ci.type = "poly", col = "blue", lwd = 2, ci.lty = 0,+      ci.col = "lightblue", main = "Species Accumulation Curves", xlab = "Number of samples",+      ylab = "Number of  species")> boxplot(all, col = "yellow", add = ...

文章 NCBI 中搜索某类基因蛋白序列技巧

...子序列： 题目：Genome-wide identification and characterization of the 14–3-3 family in Vitis vinifera L. during berry development and cold- and heat-stress response https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-018-4955-8 搜索方法演示： https://www.ncbi.nlm....