找到约 15 条结果

文章 bedtools统计序列中碱基含量

...ore the first space or tab is used.Output format:        The following information will be reported after each BED entry:            1) %AT content            2) %GC content            3) Number of As observed            4) Number of Cs observed            5) Number...

文章 GATK报错:Could not find walker with name: VariantFiltration

...OR ##### ERROR This means that one or more arguments or inputs in your command are incorrect. ##### ERROR The error message below tells you what is the problem. ##### ERROR ##### ERROR If the problem is an invalid argument, please check the online documentation guide ##### ERROR (or rerun your comma...

话题 fpkm_and_plot.R

文章 ggplot2-气泡图(控制点的大小范围)

...   2.397575 1.772599e-03   128  ko00860        Porphyrin and chlorophyll metabolism    2.574238 1.772599e-03   149  ko00062                       Fatty acid elongation    2.857551 2.316650e-03   1810 ko00630     Glyoxylate and di...

文章 NCBI下载的10X单细胞数据只有read1和read2如何读入cellranger

...True,                       help='Tab-delimited table with Run and Library_Name columns')    parser.add_argument('--force', action='store_true',                       help='Overwrite existing links/files in output directory')    return parserdef main():    parser = crea...

问题 进行bugbase运行时候老报错

...alized_otus, test_trait,  :    Error: no OTU overlap between OTU table and trait table. Execution halted

问题 微生物多样性分析课程镜像ampliseq-q2运行出错:there is no package called ‘BiocManager’

...ncountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more. Debug info has been saved to /tmp/qiime2-q2cli-err-u8wvbzh9.log 打开日志文件: Error in library(BiocManager, quietly = TRUE) : there is no package called ‘BiocManager’ Calls: .First...

问题 Fst,pi, and ROD 分析

在进行选择消除分析  fst pi ROD  分析的时候,画出的图的染色体数目中间怎么少了几条?应该有28条染色体,只画出来19条。

文章 BUSCO评估基因组组装和完整性详解

... 238     Complete BUSCOs (C)        236     Complete and single-copy BUSCOs (S)        2       Complete and duplicated BUSCOs (D)        3       Fragmented BUSCOs (F)        14      Missing BUSCOs (M)        255  ...

文章 给一个fastq ID列表,输出指定ID对应的双端fastq文件:

.../);$keep{$tmp[0]}=1;}close(IN);my$i=0;while ( my $obj1=$fq1->next_seq() and my $obj2=$fq2->next_seq() ) {my$id1=$obj1->id;my$id2=$obj2->id;#print "$id1,$id2\n";#die;if( exists $keep{$id1} or exists $keep{$id2}){$fqo1->write_seq($obj1);$fqo2->write_seq($obj2);}}$fq1->close();$fq2...

问题 基因组重测序使用qualimap 出现Exception in thread "main" java.awt.AWTError: Can't connect to X11 window server using 'localhost:18.0' as the value of the DISPLAY variable.

...am -outfile report.pdf -outformat PDF  -outdir ${bwa_qcdir}/${name}.depth_and_coverage_report done if [ $? -eq 0 ]; then        touch  ${bwa_qcdir}/ok.cucumber_bwa.qc.status     else        touch  ${bwa_qcdir}/failed.cucumber_bwa.qc.status fi 最后运行的时候  #1.比对...

文章 bioperl处理fasta和fastq序列截取反向互补翻译等等

...      -format => 'fasta');while ( my $seqobj1 = $in1->next_seq() and  my $seqobj2 = $in2->next_seq()) { # the human read-able id of the sequence my $id=$seqobj->id();  # string of sequence my $seq=$seqobj->seq();  # a description of the sequence my $desc=$seqobj->desc();  # ...

问题 RNAseq有参转录组分析差异表达分析免疫亚型之间差异表达这一环节出现以下问题

...ted. It will create various problems          with time zone changes and daylight saving time adjustments. The RTC          time is never updated, it relies on external facilities to maintain it.          If at all possible, use RTC in UTC by calling          'timedatectl set...

文章 茶树在非生物胁迫下的转录响应

...数量最多(20014),其次是organismal systems (E,13925)和genetic information (C,9452)。说明代谢相关的通路在Al响应的过程中可能被激活,参与了相关的生理过程。并且metabolism 过程可以细分为更多的代谢相关通路。 差异表达分析与GO富集 ...

文章 clinvar数据库人类疾病数据库ANNOVAR数据库使用

...dentifiers in CLNDISDB"CLNDISDB ="Tag-value pairs of disease database name and identifier, e.g. OMIM:NNNNNN"CLNDISDBINCL ="For included Variant: Tag-value pairs of disease database name and identifier, e.g. OMIM:NNNNNN"CLNHGVS ="Top-level (primary assembly, alt, or patch) HGVS expression."CLNREVSTAT...