找到约 15 条结果

问题 老师好,我在利用在SNP设计dcasp标记时得到的primers.result.xls文件为空。

...: No such file or directory Options done Output selected CAPS file name is:  CAPS_output/selected_CAPS_primers.txt ids is  [] SNP_A, SNP_B  G A CAPS found with enzyme  BstNI,PspGI,19 One nt can be changed to fit enzyme NlaIII,CviAII,FatI,132 change position and primer end postions are  2...

文章 indel vcf文件转化为SNPT输入文件

...=========Discription:        This script is used to calculate thresold for BSA using SNP-INDEX method==================================================================================================Usage:  Options:  -vcf          <str>   required        vcf list file  -o  ...

问题 LTR注释相关疑问

老师您好,我使用LTR_HARVEST_parallel、LTR_FINDER_parallel以及LTR_retriever步骤进行LTR注释,但最终打开结果文件ref.fa.mod.LTRlib.fa只有性染色体的注释结果,这是为什么?在进行LTR_retriever有一些报错如下

文章 R 包RIdeogram安装报错

...** using staged installation** R** inst** byte-compile and prepare package for lazy loading** help*** installing help indices** building package indices** testing if installed package can be loaded from temporary location** testing if installed package can be loaded from final location** testing if ...

文章 基因家族的定义和形成方式(加倍与扩张)

...具有明显的相似性,编码相似的蛋白质产物 A gene family is a set of several similar genes, formed by duplication of a single original gene, and generally with similar biochemical functions.  基因家族的形成方式(加倍与扩张): 1.染色体組多倍化( Whole...

文章 真菌 ncRNA 基因特征及坚定方法

...n non-coding RNA genes. The mitochondrial rRNA genes are single copy genes for the large and small ribosomal RNAs (rrl and rrs). These are significantly shorter than their counterpart in the nuclear genome and cannot be identified by RNAmmer(Lagesen, Hallin, Rodland, Staerfeldt, Rognes and Ussery ...

问题 请问mapman分析kegg时,总提示这个 Data does not fit mapping used (no data points mapped),please choose the appropriate mapping which matces your data

请问mapman分析kegg时,总提示这个 Data does not fit mapping used (no data points mapped),please choose the appropriate mapping which matces your data

问题 各位老师,想问一下关于LAI评估基因组 组装质量的问题

我用ltr-finder 找ltr,然后用ltr-retriever 计算LAI,基本跑通了,但是遇到点问题如下: 1 有个小警告,但是可以继续运行 2 最后结果总是ltr太少,我想知道是我的运行的命令问题还是我输入的基因组有问题呢?   3 我输入的是全...

文章 linux shell 将多行文件转换为一行

...eads argu-       ments from the standard input, delimited by blanks (which can be pro-       tected with double or single quotes or a backslash) or newlines       上面是man手册对xargs的说明。其实xargs就是对shell命令行参数限制做的一个扩展。       ...

问题 基因组比对

... proteins (77.14% of the input sequences) clustered into 27,222 groups, of which 4,292 contained sequences from all species, 3,266 were angiosperm-specific and 462 Eudicot-specific. Patterns of gene-family sharing among asterids and among woody species are shown in Fig. 1b, c. For 38,852 ash protei...

文章 蛋白质比对软件 blastp psi-blast phi-blast

...序列与蛋白序列之间的比对 Standard protein BLAST is designed for protein searches. Blastp用于确定查询的氨基酸序列在蛋白数据库中找到相似的序列。跟其它的Blast程序一样,目的是要找到相似的区域。 2,PSI-BLAST : 敏感度更高的蛋白序列...

问题 建立索引

...xt" gtfToGenePred -genePredExt $gtf unknown_refGene.txt echo "RUN CMD: retrieve_seq_from_fasta.pl --format refGene --seqfile $fa  unknown_refGene.txt --out unknown_refGeneMrna.fa" retrieve_seq_from_fasta.pl --format refGene --seqfile $fa --outfile unknown_refGeneMrna.fa unknown_refGene.txt 

文章 GATK Hard-filter 过滤变异结果推荐阈值

Hard-filter阈值探究 GATK4官网给出的推荐阈值:For SNPs: QD < 2.0MQ < 40.0FS > 60.0SOR > 3.0MQRankSum < -12.5ReadPosRankSum < -8.0  For indels: QD < 2.0ReadPosRankSum < -20.0InbreedingCoeff < -0.8FS > 200.0SOR > 10.0 查看GATK...

文章 如何绘制SNP密度图

...gical", "help",  'binsize', 's', 1, "integer", "the size of bin for SNP_density plot, optional.",  'color', 'c', 1, "character", " the colour for the SNP density, separated by ',', optional.",  'name', 'n', 1, "character", "add a character to the output file...

问题 MENA网站上传数据提示错误

...tware error: Couldn't open D:\wwwroot\MENA\ori_files\shy666..\MENAPH1.txt for writing: No such file or directory at D:\wwwroot\mena\upload_result.cgi line 95. For help, please send mail to this site's webmaster, giving this error message and the time and date of the error. 我用网站提供的...