...：
1：Only READ1 is set.
2：Only READ2 is set.
O：Either both READ1 and READ2 are set；or neither is set.
对于PE测序reads，同时指定-1 R1.fq-2 R2.fq-s singleton.fq 时，samtools会将flag1和flag2配对的序列分别输出到-1-2指定的文件，对于无法匹配的flag 1/2，...
...shed 2019 Oct 4.3. Van Calster B, Wynants L, Verbeek JFM, et al. Reporting and Interpreting Decision Curve Analysis: A Guide for Investigators.Eur Urol. 2018;74(6):796-804.4. Yan P, Li JW, Mo LG, Huang QR. A nomogram combining inflammatory markers and clinical factors predicts survival in patients w...
...ID中注释信息，避免错误：no ASV ids overlap between input FASTA and sequence abundance table
get_fa_by_id.pl $workdir/5.pick_otu_qiime/pick_de_novo_otus/otu_table_clean.txt $workdir/5.pick_otu_qiime/pick_de_novo_otus/rep_set/qiime_rep_set.fasta rep_set.farm -rf picrust2_outpicrust2_pipeli...
.../);$keep{$tmp[0]}=1;}close(IN);my$i=0;while ( my $obj1=$fq1->next_seq() and my $obj2=$fq2->next_seq() ) {my$id1=$obj1->id;my$id2=$obj2->id;#print "$id1,$id2\n";#die;if( exists $keep{$id1} or exists $keep{$id2}){$fqo1->write_seq($obj1);$fqo2->write_seq($obj2);}}$fq1->close();$fq2...
...拟电脑 Bio-Linux-8.0.7 打开一个新任务.VT-x is disabled in the BIOS for all CPU modes (VERR_VMX_MSR_ALL_VMX_DISABLED).返回 代码: E_FAIL (0x80004005)组件: ConsoleWrap界面: IConsole {872da645-4a9b-1727-bee2-5585105b9eed} 报错截图：
解决办法：
当...
...te-packages/qiime2/sdk/action.py", line 245, in bound_callable output_types, provenance) File "/share/work/biosoft/conda/Miniconda3.8/envs/qiime2-2020.8/lib/python3.6/site-packages/qiime2/sdk/action.py", line 452, in _callable_executor_ ret_val = self._callable(output_dir=temp_dir, **vie...
...sql.sock -e "show variables like 'server_id';"Warning: Using a password on the command line interface can be insecure.
+---------------+-------+
| Variable_name | Value |
+---------------+-------+
| server_id | 7 |
+---------------+-------+
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