...要获取文件句柄，只是稍有变化。
输入文件句柄：
open IN，"gzip -dc infile.gz|"
输出文件句柄：
open OUT, "| gzip > outfile.gz" or die $!;
也可以用包：PerlIO::gzip
use PerlIO::gzip; #Perl IO
open FQ,"<:gzip",file1.gz||die"$!";
open FN,">...
...，计算完FPKM后进行相关性计算并绘图，出现报错：
Error in seq.int(0, 1, length.out = n) :
'length.out' must be a non-negative number
该报错发生在选择颜色的函数colorRampPalette()上，发现是给的值出现问题，而值来自于相关性计算后的矩阵，...
...
老师好，在运行juicer时报错：
awk: calling undefined function andinput record number 1116, file /home/data/t090519/JF/20.Jui/juicer/work/splits/JF2.fastq.gz.sam source line number 298***! Failure during chimera handling of /home/data/t090519/JF/20.Jui/juicer/work/splits/JF2.fastq.gz...
必须对bam文件进行默认情况下的排序后，才能进行index。否则会报错。
Usage: samtools index <in.bam> [out.index]
Options: -b Generate BAI-format index for BAM files[default] samtools index -b xx.bam 建立索引后将产生后缀为.bai的文件...
... Hu, Wei-Hua Chen, Evolview v3: a webserver for visualization, annotation, and management of phylogenetic trees, Nucleic Acids Research,(2019) (https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkz357/5494715)
1.新建上传自己的tree文件：
Upload tree control iconProject name tex...
...;
把文件的名字（前缀）赋给每个读取到的数据
> for (i in 1:length(ABC)){+ assign(make.names(gsub(".txt","",tem[i])),data.frame(ABC[[i]]))+ }> A V1 V21 A 1> B V1 V21 B 2> C V1 V21 C 3
筛选出的deg的基因emsembl id无法进行转成entrezid，说不存在这些id
Error in .testForValidKeys(x, keys, keytype, fks) :
None of the keys entered are valid keys for 'ENSEMBL'. Please use the keys method to see a listing of valid arguments.
Calls: bitr
Execution halted
...ght (c) 2005, Carter T. Butts, University of California-IrvineFor citation information, type citation("sna").Type help(package="sna") to get started.Error in `rownames<-`(x, value) :length of 'dimnames' [1] not equal to array extentCalls: row.names<- -> row.names<-.default -> rownames...
...ary to load it. Artifact has type 'SampleData[JoinedSequencesWithQuality]' and format 'SingleLanePerSampleSingleEndFastqDirFmt'
See above for debug info.
/usr/lib64/R/library/cpp11/include/cpp11/protect.hpp: In member function ‘constexpr R cpp11::protect::function<R(A ...)>::operator()(A ...) const [with R = SEXPREC*; A = {SEXPREC*, SEXPREC*}]’:/usr/lib64/R/library/cpp11/include/cpp11/protect.hpp:186:5: warning: control reaches end of non-v...
...niq -u > c # c 是 a - b
awk常用命令
awk '{ sum += $2 } END { print sum }' myfile #这个命令将计算myfile文件的第二列的总和并打印出来。awk '{ print $2, $1 }' myfile | sort -n #这个命令将打印出myfile文件的第二列和第一列，并将它...
There was a problem importing manifest:
manifest is not a(n) PairedEndFastqManifestPhred33V2 file:
Filepath on line 1 and column "forward-absolute-filepath" could not be found ($PWD/home/qiime2/Public/data/dyj/HS15_1.fq.gz) for sample "HS15".