...信息 ### Failed to create bus connection: No such file or directory
Warning message:
###Using `size` aesthetic for lines was deprecated in ggplot2 3.4.0.
###Please use `linewidth` instead.
是否是正确的 以及输出文件仅有4个 与课程视频中的预期不同 所得到的...
...，我用了python脚本
import gzipimport argparsedef extract_gene_names(input_fastq_gz, output_file): with gzip.open(input_fastq_gz, 'rt') as fastq_gz, open(output_file, 'w') as out_file: line_count = 0 for line in fastq_gz: line_count += 1 ...
...==============================================| 100%
To get the following information please change the clinical.info argument
=> new_tumor_events: new_tumor_event
=> drugs: drug
=> follow_ups: follow_up
=> radiations: radiation
Parsing follow up version: follow_up_v1.0
|...
Error in validObject(object = object) :
invalid class “Assay5” object: Layers must be two-dimensional objects
原因是batch分组中有些样本细胞数量太少 为1 造成：
seurat subset之后再聚类报错：
...型对数据的拟合优度的一种统计检验方法；AIC值（Akaike Information criterion, AIC）是指Kullback-Leibler (K-L) 距离的估计值，K-L距离定义为一个模型被另一个模型替代后信息的丧失。因此，AIC值较小的模型更好；BIC（Bayesian Information Criteri...
...ction2 二代组装：spades assemblies3 混合组装：assembling contigs and long reads with miniasm4 比对：aligning reads5 搭桥：building long read bridges6 抛光：polishing assembly with pilon
组装结果
参考：https://www.jianshu.com/p/6ac7762a8f3a
The virtual machine 'Bio-Linux-8.0.7' has terminated unexpectedly during startup with exit code 1 (0x1). More details may be available in 'D:\VirtualBox VMs\Bio-Linux-8.0.7\Logs\VBoxHardening.log'.