找到约 15 条结果

文章 比较3种差别表达方法对TCGA转录组数据的分析

...用“TCGA-CHOL” 癌症的转录组数据进行分析,该数据集有45份样本,其中36份癌症组装,9份癌旁组织。分析采用edgeR, DESeq2, limma 进行差异分析。 1. 上调基因绘制韦恩图 2 下调基因绘制韦恩图 3. 差异表达基因绘制韦恩图 从...

问题 泛基因家族分析cds提取出现killed

...ot 466M Dec 20 16:48 AD4.longest_isoform.gff3 [root@c4bf00d21ba0  09:45:29 /work/01.cotton_data_prepare]# /share/work/biosoft/TransDecoder/latest/util/gff3_file_to_proteins.pl --gff3  ./${ind}.AD4.longest_isoform.gff3 --fasta ./${ind}AD4_JGI.genome.fa.gz --seqType CDS >./${ind}.cds.fa Kil...

文章 最新基因家族分析文献详解-冬枣扩展蛋白基因家族分析-11月8日接收

...Genome‑wide identifcation, characterization, and expression analysis of the expansin gene family in Chinese jujube (Ziziphus jujuba Mill.) 的冬枣中扩展蛋白家族(expansin gene family)分析文章,一起来看一看,基因家族需要做哪些分析就能发2区的期刊!原文献...

文章 利用awk 对某一列数据取对数

...行计算,第一行直接输出或跳过皆可) [raop@cluster  13:45:13 /share/nas1/raop/project]$cat test.xlsGene    FCA       2B       1.5C       0.3D       10 对数据第一行NR==1 变形输出 Gene\tlog2(FC) 对数据其他行NR!=1 第二列进行...

文章 人类miRNA-mRNA 与 miRNA-lncRNA 靶向数据库总结

The experimentally verified miRNA-mRNA pairs were downloaded from starBase v3.0 (18), DIANA-Tarbase v8 (19), miRTarbase (v7.0) (20) and miRSponge (21) databases, while the experimentally validated miRNA-lncRNA pairs were extracted from starBase v3.0 (18), LncACTdb2.0 (22), DIANA-LncBase Experimental...

文章 6个转录组发了BMC genomics,人家是怎么写的?

...12日见刊的题为“Transcriptomes analysis reveals novel insight into the molecular mechanisms of somatic embryogenesis in Hevea brasiliensis”转录组文章,内容难易适中,很有代表性,那就让我们一起看一看,6个转录组样本,每个重复3次,是如何发到BMC Ge...

问题 使用circos对共线性结果进行美化,染色体的标签名字发生重叠,调整了参数也不起效,该如何调整?

... label_parallel=yes label_radius=dims(image,radius)-60p label_size=45 radius=0.5r #设置半径,以免基因名称过长超出显示范围 show_label=yes <spacing> default=0.005r </spacing> stroke_color=dgrey stroke_thickness=2p thickness=0.03r</...

问题 在做叶绿体组装时,出现了如下错误,该如何解决呢

...//github.com/Kinggerm/GetOrganelle/wiki/FAQ      66 Secondly, check if there are open/closed issues related at https://githu      67 If your problem was still not solved,      68   please open an issue at https://github.com/Kinggerm/GetOrganelle/issue      69   please provide the...

问题 用GATK call SNP时报错

...ease index all input files: samtools index SRR3274663.new.sorted.bam Set the system property GATK_STACKTRACE_ON_USER_EXCEPTION (--java-options '-DGATK_STACKTRACE_ON_USER_EXCEPTION=true') to print the stack trace. 这是我的命令:gatk --java-options "-Xmx20g -Djava.io.tmpdir=./tmp" HaplotypeC...

问题 Error running system command:

...gt; NOTICE: Finished reading 5099177 lines from VCF file NOTICE: A total of 5099109 locus in VCF file passed QC threshold, representing 5099109 SNPs (3715827 transitions and 1383282 transversions) and 0 indels/substitutions NOTICE: Finished writing allele frequencies based on 2365986576 SNP genot...

文章 seqkit序列处理神器的常用命令

...a/q file manipulation. PloS One 11, e0163962, doi: https://doi.org/10.1371/journal.pone.0163962 软件发布页:https://github.com/shenwei356/seqkit/releases 帮助文档:https://bioinf.shenwei.me/seqkit/ 参考:https://www.cnblogs.com/huangyinger/p/10421805.html

文章 轻松下载k8s.gcr.io,gcr.io,quay.io镜像

...下所示:[root@k8scloude2 ~]# docker pull quay.io/kubevirt/virt-api:v0.45.0 #换成 [root@k8scloude2 ~]# docker pull quay.mirrors.ustc.edu.cn/kubevirt/virt-api:v0.45.0

问题 老师,请问CGVIEW中的gene.blast文件格式

...,望你解答。谢谢! 报错和blast文件如下: Cannot parse the #PROGRAM field in the BLAST results file ./YC_bacteria_cgview/blast.txt at ./scripts/cgview_xml_builder/cgview_xml_builder.pl line 4716, <INFILE> line 0.000000. #PROGRAMquery_id    match_id    match_descripti...

文章 bam文件转换fastq

...eating paired FASTQ.        -tags   Create FASTQ based on the mate info                in the BAM R2 and Q2 tags.Tips:        If you want to create a single, interleaved FASTQ file        for paired-end data, you can just write...

问题 WGCNA-MAD

...################################## input data########################## # The following setting is important, do not omit. options(stringsAsFactors = FALSE) #读取数据,删除第一列GeneSymbol datExpr = read.table(expr_file,header=T,row.names=1,comment.char = "",check.names=F) datExpr = d...