找到约 15 条结果

文章 错误：perl: warning: Please check that your locale settings: LANGUAGE = (unset), LC_ALL = (unset), LC_CTYPE = "UTF-8", LANG = "en_US.UTF-8"

...~/.bashrc 或 ~/.bash_profile 文件加上下面的参数， # Setting for the new UTF-8 terminal support in Lion export LC_CTYPE=en_US.UTF-8 export LC_ALL=en_US.UTF-8 如果是使用zsh的mac，则是修改~/.zshrc文件 # Setting for the new UTF-8 terminal support in Lion LC_CTYPE=en_US.U...

问题 电脑内存不能升级，运行Rstudio内存一直上升，请问还有其他方法解决这个问题吗。

...ct <- "TCGA-LUAD" > data_category <- "DNA Methylation" > platform <- "Illumina Human Methylation 450" > sample_type <- "Primary solid Tumor"    > legacy <- FALSE > # 工作目录如果不存在，则创建目录 > if( !file.exists(work_dir) ){ +   if( !dir...

问题 用W11安装docker后，docker search omicsclass出错

用W11安装docker后，docker search omicsclass出错 Error response from daemon: Get "https://index.docker.io/v1/search?q=omicsclass&n=25": dialing index.docker.io:443 container via direct connection because  has no HTTPS proxy: connecting to index.docker.io:443: dial tcp 108.160.172.1:443: ...

问题 GO富集气泡图和柱状图一样报错

... = what, sep = sep, quote = quote, dec = dec,  :   number of items read is not a multiple of the number of columns Error in `[<-.data.frame`(`*tmp*`, GO$Pvalue == 0, "Pvalue", value = 1e-15) :    missing values are not allowed in subscripted assignments of data frames Calls: [<- -> ...

问题 利用perl脚本提取基因上游的1500bp（指定位置）序列时提示出现Incorrect parameters to subseq的错误

...file => "$ARGV[0]",                                  -format => 'Fasta');$out = Bio::SeqIO -> new(-file => ">$ARGV[2]",                                  -format => 'Fasta');my %keep=() ;open IN,"$ARGV[0]" or die "$!";my%ref=();while ( my $seq = $in...

问题 TCGAbiolinks下载“Copy Number Variation”在提取矩阵时报错（在下载miRNA和甲基化数据时也遇到这个问题）， expdat变量不是SummarizedExperiment这个类型，导致assay(expdat)不能提取，请问怎么解决

...unction (classes, fdef, mtable)  :   unable to find an inherited method for function ‘assay’ for signature ‘"spec_tbl_df", "missing"’

文章 Python学习-利用Python对植物基因组注释文件（gff3格式）进行处理

...字 list_two = [] chr_name = [] de_list = ('#','M','P','s') # 需要修改 for line in input_file: if line.startswith(de_list): continue list_one = line.strip().split() if list_one[2] == 'mRNA': # gene_id = list_one[8].split(';')[2] # 需要修改 gene_id = list_o...

文章 人类癌症数据库突变注释

...ions from cancer and the frequency of occurence in each subtype of cancer. For more updated cosmic, see instructions below on how to make them.icgc21: International Cancer Genome Consortium version 21 mutations.nci60: NCI-60 human tumor cell line panel exome sequencing allele frequency data

文章 DEG_limma.R 差异基因分析limma

...nal,default:1] -X x.lab, --x.lab x.lab the label for x axis[optional,default:log2FC] -Y y.lab, --y.lab y.lab the label for y axis[optional,default:-log10(FDR)] -T title, --title title the label for main title[optional,defa...

问题 老师您好，我在运行OrthoFinder2时，出现下面报错，请老师解答

..., line 35, in __init__ self.non_gap_pos[-1].extend([current_length + i for i,c in enumerate(line) if (c != "*" and c != "-")]) WARNING: list index out of range WARNING: list index out of range File "/share/work/biosoft/OrthoFinder/OrthoFinder_v2.5.4/scripts_of/parallel_task_manager.py", line 2...

文章 二倍体杂合基因组组装策略

...anu策略 参考资料： Purge Haplotigs: allelic contig reassignment for third-gen diploid genome assemblies | BMC Bioinformatics | Full Text (biomedcentral.com) HapSolo: an optimization approach for removing secondary haplotigs during diploid genome assembly and scaffolding | BMC Bioinformat...

问题 使用悟空云平台进行代谢组数据矫正时报错

其余五种归一化方式均正常，只有QC-RLSC报错显示error in evaluating the argument X in selecting a method for function "t: NA NaNI nf in foreign function call (arg 5)

问题 getorganelle组装线粒体出现问题

老师您好，在组装线粒体基因组时出现了 For Ge1tOrganelle1_out/1-illumina_1.fq.gz.fastq, only top 31563574 reads are used in downstream analysis.这个问题，组装结果中只有一条8000bp左右的序列，请问怎么设置参数才能不 only top reads进行后续分析，而...

问题 基因组注释：10.PASA_update，根据fa和gff文件生成pep或者cdna

基因组注释：10.PASA_update，根据fa和gff文件生成pep或者cdna文件，一直显示： No such file or directory at /share/work/biosofTransDecoder/TransDecoder-v5.5.0/util/../PerlLib/GFF3_utils2.pm line 37, <$filehandle> line 112.

问题 关于图形泛基因组中的Minigraph-Cactus的问题。

...tus的脚本中提到了，所生成的三种文件，其中 clip graph: This is the default graph. Stretches of sequence >10kb that were not aligned to the underlying SV/minigraph are removed. "Dangling" nodes (ie that don't have an edge on each side) that aren't on the reference path are also r...