找到约 15 条结果

文章 空间转录组数据读取

...ject_name = "SeuratProject", filter_matrix = TRUE) { # Function to read the count matrix and create Seurat object read_count_matrix = function(data_dir) { counts = Read10X(paste0(data_dir, "/filtered_feature_bc_matrix")) seurat_object = CreateSeuratObject(counts = counts, assay =...

文章 两个诺贝尔奖强强联合--CRISPR基因编辑的ESC/iPSC应用于再生和个性化医学

..., January 14, 2020. Gene Correction of LGMD2A Patient-Specific iPSCs for the Development of Targeted Autologous Cell Therapy. Molecular Therapy, Vol. 27 (12) December 2019. 文章是源井生物原创,转载请注明!

文章 Biopython之序列输入

...ANAC011 description:NAC011 [Source:UniProtKB/TrEMBL;Acc:A0A178WL27] Number of features: 0 Seq('ATGGTAGGATCATTTTTACCACCTGGTTTCAGATTTTATCCAACAGATGAAGAA...TAA', SingleLetterAlphabet()) 此外,我们在网易云课堂上有各种教学视频,有兴趣可以了解一下: 1. 文章越来越...

文章 kaks和dnds是什么?

...以后的高级篇中,我们会深入探讨这些问题。 Hurst L D. The Ka/Ks ratio: diagnosing the form of sequence evolution[J]. TRENDS in Genetics, 2002, 18(9): 486-487. Navarro A, Barton N H. Chromosomal speciation and molecular divergence--accelerated evolution in rearranged chromosomes...

问题 老师您好,我在文件共享时出现不能共享的问题,具体操作如下: biolinux sudo mount -t vboxsf windows-share /home/manager/share 结果为:/sbin/mount.vboxsf: mounting failed with the error: No such device,该怎么解决,谢谢

问题 老师好,我在运行比较基因组学11.time_tree_BV脚本的时候,报错Error: Only bounds for the root age are implemented,修改了几次化石时间节点也没有成功,请问这个是我的化石节点选的有问题么?

问题 使用Launch_PASA_pipeline.pl 脚本报错

...RNING] Indexing parameters (-k, -w or -H) overridden by parameters used in the prebuilt index.[M::main::6.314*1.00] loaded/built the index for 152 target sequence(s)[M::mm_mapopt_update::7.619*1.00] mid_occ = 834[M::mm_idx_stat] kmer size: 15; skip: 10; is_hpc: 0; #seq: 152[M::mm_idx_stat::8.519*1.0...

问题 在docker中用cleandata与宿主序列比对得到sam文件这一步出现错误,

...tie.map.log  # Filter and output as SAM(or BAM),-f 4 to Remove reads from the alignment,  # if you want to keep the -F on the alignment (12 for both ends), add -b before the output to output in BAM format samtools view --threads 6 \ -f 12 -h \ $workdir/1.host_del_and_qc/cleandata/${i}.bowtie.sam \...

文章 R语言如何绘制MA图

... length(args) != 2 ) {    print(args)    usage()    stop("the length of args != 6")}plot_MA <- function(log10exp=NULL, log2FC=NULL, FDR=NULL, Significant=NULL,        xlab="log10(FPKM)", ylab="log2(FC)", main="MA plot") {    # check args    # chec...

文章 perl语言标量SCALAR

...符 取消字符含义:   取消$的变量标识       print "the \$var is $var \n";   取消双引号"             print "A quote \" in a string \n";   取消反斜杠\            print "A quote \\ in a string \n"; \nnn (n表示数字)   八进制表示的 ACSII...

问题 有参转录组分析

..._category, De... dbl  (2): evalue, score ℹ Use `spec()` to retrieve the full column specification for this data. ℹ Specify the column types or set `show_col_types = FALSE` to quiet this message. Error in file(file, "rt") : cannot open the connection Calls: read.table -> file In addit...

问题 我运行遗传群体进化的fst_pi_select_sweep.r这个脚本一直报错Error in dev.off() : cannot shut down device 1 (the null device),将dev.off()语句改为while (!is.null(dev.list()))  dev.off()依旧报错,怎么解决?

文章 Vennerable包的安装

...ors.tuna.tsinghua.edu.cn/CRAN/") library(devtools) ###Use reshape2 instead of reshape install_github("js229/Vennerable") 1、最好先安装依赖的包,以免影响软件安装 2、安装devtools  并加载,以便能够使用安装命令install_github 3、利用install_github 安装 Ven...

问题 运行GDCprepare(query, save=FALSE)时错误

... FALSE) Error in `vectbl_as_col_location()`: ! Can't subset columns past the end. i Locations 2, 3, and 4 don't exist. i There is only 1 column. Run `rlang::last_error()` to see where the error occurred. There were 50 or more warnings (use warnings() to see the first 50) > rlang::last_erro...

文章 单细胞转录组数据标准化:

...达对基因方差的影响。 https://www.biorxiv.org/content/10.1101/2021.08.18.456898v1.full