找到约 15 条结果

问题 Error in UseMethod("groupOTU") : no applicable method for 'groupOTU' applied to an object of class "NULL" Calls: groupOTU Execution halted

各位老师,我在绘制进化树的过程中,使用命令ggtree.r -t fasttree.nwk -f $GROUP -g Group -n fasttree,运行后报错,出现下图报错,请问老师,我应该怎么处理呢?

问题 WGCNA分析中,导入txt的性状数据时(数据为12行样本,10列性状数据),报错Error in scan(file = file, what = what, sep = sep, quote = quote, dec = dec, : line 1 didot have 12 elements。但是数据如果是12行样本,8列性状,不会报错

...r=T,comment.char = "",check.names=F)但是报错Error in scan(file = file, what = what, sep = sep, quote = quote, dec = dec,  :    line 1 did not have 12 elements。但是数据如果是12行样本,8列性状,就不会报错。谢谢

文章 GEO数据与WGCNA--挖掘胶质瘤共表达网络的关键模块与通路

...富集结果显示:hsa04510(Focal adhesion)和hsa04610(Complement and coagulation cascades)显著富集, 这两个pathway极有可能是胶质瘤的生物标记。 文章以现有数据(GEO)为基础,进行WGCNA模块分析过程,并对模块基因进行富集分析,重点挖...

文章 IF=5|干眼症微生物多样性分析案例解析

...投入大产出。参考文献:Qi Y, Wan Y, Li T, et al. Comparison of the Ocular Microbiomes of Dry Eye Patients With and Without Autoimmune Disease. Front Cell Infect Microbiol. 2021;11:716867. Published 2021 Sep 22. doi:10.3389/fcimb.2021.716867

问题 合并选择区域时出现错误,请教一下,要怎么解决呢?是不是把前面的- 删掉

...i - >fst_top0.5_selected_region.txt Error: Sorted input specified, but the file - has the following out of order record hic_scaffold_13100014100002.170817908590890.004903580.00525442 -0.0996961085700556FS_D selected region

问题 群体遗传学GWAS课程docker很多命令无法运行

...lter/all.varFilter.vcf.gz -SubPop popid.txt -MaxDist 500 -OutStat ld.stat the Number of subPop samples[found in VCF] is 0 sub Group Population szie is too small, SubGroup sample size: 0 ##begin pair-wise R^2 cal. after filter Remain SNP Number :     0 #% number bin is        1 1%......--...

文章 barplot()R语言绘制状图

...101467081Amino Acid Metabolism0.0990784610.1023070980.098133001Replication and Repair0.0640276080.081496790.077107142Energy Metabolism0.0630605790.0669996830.055660175Translation0.0486662250.0525558320.048639947Poorly Characterized0.0501483020.0474659650.049391479Metabolism of Cofactors and Vitamins...

问题 在提取1500bp序列做顺式作用元件检测时,使用tiquxulie.pl这个脚本,开始时显示out of memory,我把虚拟内存提高到了4G,显示zsh:killed,得到的结果还是空的,请问老师这个问题怎么解决呢?

谢谢老师。

问题 用Tassel转vcf为hapmap格式文件

...1B  Pos:711831      Name:S1B_711831 Variants:C/T    MAF:NaN Ref:C and Position      Chr:7A  Pos:736691630   Name:S7A_736691630  Variants:T/C     MAF:NaN Ref:T out of order.

问题 TCGA下载数据保存

下载FPKM的时候,保存结果时出现Error in read_connection_(con) : Evaluation error: error reading from the connection. In addition: Warning message: In (function (con, what, n = 1L, size = NA_integer_, signed = TRUE,  :   invalid or incomplete compressed data

问题 在运行core_pan_gene_curve.r的时候报错

...vironment value of R_HOME --> Q&A for bioinformatics, please visit the website: https://www.omicsclass.com/ --> R beginners ? I suggest your  learning  R language: https://study.omicsclass.com/index Attaching package: ‘dplyr’ The following objects are masked from ‘package:s...

问题 TCGAbiolinks GDCprepare使用问题 提示找不到文件?

... data.category = "Transcriptome Profiling", data.type = "Gene Expression Quantification", workflow.type = "HTSeq - Counts") GDCdownload(query) data <- GDCprepare(query) 下载成功了,自动生成了GDCdata文件夹,但它里面是空的,...

文章 BSA分析之MutMap分析原理详解!

...tructed by replacing nucleotides in Nipponbare with those of Hitomebore at the 124,968 SNP positions identified between the two cultivars by alignment of 12.25 Gb of Illumina Hitomebore short reads to the Nipponbare reference genome MutMap适用条件: 1. 一般为隐性突变,最好是EMS诱...

问题 有参转录组分析

...rieve the full column specification for this data. ℹ Specify the column types or set `show_col_types = FALSE` to quiet this message. Error in file(file, "rt") : cannot open the connection Calls: read.table -> file In addition: Warning message: In file(file, "rt") :   cannot open file 'NA...

文章 NCBI下载的10X单细胞数据只有read1和read2如何读入cellranger

...分; cellranger命名规则 [Sample Name]_S1_L00[Lane Number]_[Read Type]_001.fastq.gz # 其中Read Type# I1: Sample index read (optional)# R1: Read 1# R2: Read 2 NCBI下载的数据,为双端reads,不符合cellranger mkfastq出来的命名规则,后续cellranger count 无法读入,...