问题 老师，这是在组学大讲堂找的三个物种做比较基因组学分析的一个脚本，运行显示Can't find string terminator " Usage End." anywhere before EOF at ../script/mcscan.filter.pl line 13.的错误，请老师看下这个脚本，谢谢老师

...name dirname);use Cwd qw(abs_path);#get optsmy %opts;GetOptions(\%opts, "in=s", "out=s","id1=s","id2=s","h");if(!defined($opts{id2}) || !defined($opts{id1}) || !defined($opts{in}) || !defined($opts{out}) || defined($opts{h})){ print <<" Usage End."; Usage Forced pa...

问题 getGEO函数不能下载，提示Error in open.connection(x, "rb") : cannot open the connection，谢谢！

文章 qiime2自己建立分类器 greengene

... 这里以V3-V4区引物为例： 338F (5′-ACTCCTACGGGAGGCAGCAG-3′) and. 806R (5′-GGACTACHVGGGTWTCTAAT-3′) # 下载数据库文件(greengenes) wget -c ftp://greengenes.microbio.me/greengenes_release/gg_13_5/gg_13_8_otus.tar.gz # 解压 tar -zxvf gg_13_8_otus.tar.gz # 使用rep...

问题 基因组重测序，变异结果注释的时候出现了Error: invalid record found in annovar outputfile

输入的命令，出现的错误，产出的文件如上。感谢各位大佬。

问题 构建进化树报错

构建进化树时报错：Oops! MEGA has encountered an error and cannot continue with this analysis:Some pairwise distances could not be estimated. For example, between sequences 25 and 12.这是为什么啊？

问题 问一下老师们，提取成对基因的时候得到结果文件总是为空是什么原因，下面是我的输入文件perl ../script/KAKS_SHAIXUAN.pl -in1 WRKY_cds_confirmed.fa.fai -in2 WRKY_cds_confirmed_blast.out -out duplication_gene.out就是这条命令。

问题 Linux服务器上，运行提取结构域的脚本的时候，出现未安装Bio/SeqIO.pm in @INC的错误，请问如何解决

Linux服务器上，运行提取结构域的脚本的时候，出现未安装Bio/SeqIO.pm in @INC的错误，请问如何解决，这个问题之前有提过，但是没有人回答，有人只是指出可以安装docker，但是需要root用户安装，没有root权限的用户怎么办

文章 CentOS 7 yum安装报错

...org; Unknown error" One of the configured repositories failed (Unknown), and yum doesn't have enough cached data to continue. At this point the only safe thing yum can do is fail. There are a few ways to work "fix" this:     1. Contact the upstream for the repository and get them to fix the pr...

问题 pip is configured with locations that require TLS/SSL, however the ssl module in Python is not available

python 编译之后报错： pip is configured with locations that require TLS/SSL, however the ssl module in Python is not available

文章 Metadata CRC error detected at xfs _ bmbt _ read _ verify+0x12/0xb0 [xfs], xfs _ bmbt block 0xc9132f8 [ 4.863216] XFS(dm-0): Unmount and run xfs _ repair

...x12/0xb0 [xfs], xfs _ bmbt block 0xc9132f8 [ 4.863216] XFS(dm-0): Unmount and run xfs _ repair [ 4.863257] XFS(dm-0): First 128 bytes of corrupted metadata buffer: [ 4.863311] 00000000: 42 4d 41 33 00 00 00 19 ff ff ff ff ff ff ff ff ff BMA3… [ 4.863375] 00000010: ff ff ff ff ff ff ff ff 00 00...

问题 GWAS筛选阈值设置

For SNPs, we set the thresholds as −log10(P value) = 6, 6, and 4 for litter size, numbers of horns and nipples, respectively. 请问，这种自选的阈值，怎么选择呢？为什么有的是6有的是4呢？

文章 BSA分析中QTL-seq 分析：95% 99%阈值置信区间是如何计算的

... ####################################Arg<-commandArgs(TRUE)###########input###############################################individual_analysis<- c(Arg[1])reprication<-c(Arg[2])filter_value<-c(Arg[3])population_structure<-c(Arg[4]) #RIL or F2depth_analysis<-c(1:300)###########input#...

问题 circos绘制基因组共线性图绘图命令运行问题

...onfiguration files work, create the example image, whose   configuration and data are found in example/. From the Circos distribution   directory,       cd example       ../bin/circos -conf ./circos.conf   or use the 'run' script (UNIX only).   Configuration files are describ...

文章 TCGAbiolinks 错误 Error in value[[3L]](cond) : GDC server down, try to use this package later

 在采用TCGAbiolinks 下载TCGA数据时，有时候会出现“Error in value[[3L]](cond) :    GDC server down, try to use this package later”的错误。 这个错误一般不会出现，除非GDC的网站有问题，检测方法有两种： 1.  在R中运行如下命令： > Genom...

问题 omicsclass/pan-genome:v1.1镜像OrthoFinder.py报错

运行动植物泛基因组教程02.gene_family.sh命令如下：orthofinder.py -f pep \ -S diamond \ -M msa \ -A muscle \ -T fasttree \ -t 10 \ -a 2 报错如下：Best outgroup(s) for species tree---------------------------------2024-11-15 10:50:56 : Starting STRIDEERROR: Species tree inf...