gatk VariantFiltration : --filter-expression 显示 Input /work/my_reseq/4.snp_indel/var_qc2/BSA_no_filter.vcf must support random access to enable queries by interval. If it's a file, please index it using the bundled tool IndexFeatureFile该怎么处理呀
bedtools软件中的nuc函数工具可以统计序列碱基含量,其具体用法如下:
Tool: bedtools nuc (aka nucBed)Version: v2.25.0Summary: Profiles the nucleotide content of intervals in a fasta file.Usage: bedtools nuc [OPTIONS] -fi <fasta> -bed <bed/gff/vcf>Options:...