话题 Error in newCountDataSet

问题 Error correction and assembling warnings: * 0:12:28.935 6768M / 13G WARN General (kmer_coverage_model.cpp : 327) Valley value was estimated improperly, reset to 16

这问题有什么解决办法吗

问题 Error correction and assembling warnings: * 0:12:28.935 6768M / 13G WARN General (kmer_coverage_model.cpp : 327) Valley value was estimated improperly, reset to 16

请问您这个谷值问题怎么解决的

问题 Error in seq.default(grps[start] + 1, grps[start + 1] - 1) : 'from' must be of length 1此外: Warning messages: 1: In file(con, "r") : InternetOpenUrl失败:’不能连接到吊销服务器，或者未能获得最终响应。' 2: In readLines(...) : 读'http://bioconductor.org/config.yaml'时最后一行未遂

在rstudio中运行 BiocManager::install("TCGAbiolinks")，迟迟无法下载数据，并显示上方错误，请问怎么解决

文章 indel vcf文件转化为SNPT输入文件

...                     ) or &USAGE;&USAGE unless ($infile and $out);sub USAGE {#        my $usage=<<"USAGE";Program: $0Version: $version==================================================================================================Discription:        This script is u...

问题 vcftools过滤除了等位基因选项外，其他参数通通没用

...参数过滤： vcftools --gzvcf all.varFilter.vcf.gz --recode --recode-INFO-all --stdout \     --maf 0.05  --max-missing 0.4  --minDP 4  --maxDP 1000  \     --minQ 30 --minGQ 80 --min-alleles 2  --max-alleles 2 |gzip - > all.clean.vcf.gz 结果是After filtering, kept 0 out of a pos...

用户 Man in the Mirror

用户 IN0vation

问题 kneaddata做质控的时候，服务器卡死了，用trimmomatic+bowtie2分开跑是可以的吧

... "$datafile"/{}_1.fq.gz "$datafile"/{}_2.fq.gz \   "$workdir"/1.host_del_and_qc/kneaddata/{}_forward_paired.fq.gz "$workdir"/1.host_del_and_qc/kneaddata/{}_forward_unpaired.fq.gz \   "$workdir"/1.host_del_and_qc/kneaddata/{}_reverse_paired.fq.gz "$workdir"/1.host_del_and_qc/kneaddata/{}_reverse_...

文章 快速解读：3-5分的蛋白质组学文章思路

iTRAQ (Isobaric tag for relative and absolute quantitation，iTRAQ）和TMT（Tandem Mass Tag）是近年来应用最广泛的差异蛋白质组学技术，它们均采用体外标记的方法，利用同位素试剂标记蛋白质酶解后产生的多肽，对两个或多个样本，在全蛋白质...

问题 samtools faidx: different length in sequence

您好! 您在2020年8月用samtools获取染色体长度时有一个different line length in sequence contig1的报错，这个问题解决了嘛？ 我最近也遇到了相同的问题

问题 扩增子分析：qiime 聚类生成OTU-denovo方法分析报错

...lib/python2.7/site-packages/qiime/workflow/util.py", line 122, in call_commands_serially     raise WorkflowError(msg) qiime.workflow.util.WorkflowError: *** ERROR RAISED DURING STEP: Assign taxonomy Command run was:  assign_taxonomy.py -o pick_de_novo_otus/uclust_assigned_taxonomy -i pick_...

问题 dadi

...by the 14 tested models (Figs. 2A, S1), with a log‐likelihood of 141.756 and an AIC of 1354.21 (Table 1). Parameter estimates inferred that the divergence time of LO and LO was 0.61 Ma (95% CIs: 0.58–0.65), and the two diverged species had a second contact at 7109 years ago (95% CIs: 6633–7585...

文章 monocle2 monocle2 igraph 报错

monocle2 igraph 报错 Error:! `nei()` was deprecated in igraph 2.1.0 and is now defunct.ℹ Please use `.nei()` instead. --- Backtrace:▆ 1. ├─monocle::orderCells(cds) 2. │ └─monocle:::project2MST(cds, project_point_to_line_segment) 3. │ ├─V(dp_mst)[suppressWarnings(nei(c...

问题 请问大佬，做转录组分析时，fpkm_and_plot.R分析数据时，出现错误Error: Aesthetics must be either length 1 or the same as the data (100276): x，这个怎么解决呀？