问题 扩增子分析：qiime 聚类生成OTU-denovo方法分析报错

...lib/python2.7/site-packages/qiime/workflow/util.py", line 122, in call_commands_serially     raise WorkflowError(msg) qiime.workflow.util.WorkflowError: *** ERROR RAISED DURING STEP: Assign taxonomy Command run was:  assign_taxonomy.py -o pick_de_novo_otus/uclust_assigned_taxonomy -i pick_...

文章 wgd syn 报错 KeyError: 6.0

...ack (most recent call last):   File "/share/work/biosoft/python/Python3/bin/wgd", line 33, in <module>     sys.exit(load_entry_point('wgd==1.2', 'console_scripts', 'wgd')())   File "/share/work/biosoft/python/Python-v3.9.16/lib/python3.9/site-packages/click/core.py", line 1130, in __cal...

文章 VirtualBox 中练习安装linux系统centos7

...Universityhttp://mirrors.tuna.tsinghua.edu.cn/centos/University of Science and Tech of Chinahttp://mirrors.ustc.edu.cn/centos/Zhejiang Universityhttp://mirrors.zju.edu.cn/centos/ 清华的镜像地址：https://mirrors.tuna.tsinghua.edu.cn/centos/7.9.2009/isos/x86_64/  版本说明 CentOS-7-x8...

问题 单细胞测序数据差异基因KEGG富集分析

...ENE"     "UNIPROT"     'select()' returned 1:1 mapping between keys and columns Reading KEGG annotation online: Reading KEGG annotation online: --> No gene can be mapped.... --> Expected input gene ID:  --> return NULL... NULL Error in (function (classes, fdef, mtable)  ...

问题 请问request returned Internal Server Error for API route and version http://%2F%2F.%2Fpipe%2Fdocker_engine/v1.24/containers... check if the server supports the requested API version 这个问题该怎么解决

...containers中出现 request returned Internal Server Error for API route and version http://%2F%2F.%2Fpipe%2Fdocker_engine/v1.24/containers/025fef2a3e783d8c473c644d58e2ffd31be5f157743e797b0649966a18031dfc/json, check if the server supports the requested API version

问题 boxplot画图提示“Error in oldClass(stats)

问题 WGCNA分析过程中做样品聚类树与形状热图时遇到问题

做性状热图的那一步时，出现了： Error in numbers2colors(datTraits, signed = FALSE) :    'lim' must be finite. In addition: Warning messages: 1: In FUN(newX[, i], ...) : no non-missing arguments to min; returning Inf 2: In FUN(newX[, i], ...) : no non-missing arguments to min; re...

问题 为什么docker下载不了第三方镜像？显示这样Using default tag: latest What's next: View a summary of image vulnerabilities and recommendations → docker scout quickview biocontainers/samtools Error response from daemo

文章 亚型划分比较波浪图（桑基图）绘制

...y of Cancer上的影响因子为13.75的文献《m6A modification patterns and tumor immune landscape in clear cell renal carcinoma》中，作者利用桑基图来描绘m6A簇、免疫亚型和m6A score的变化（B图）。 桑基图的绘制 为了使大家能更简便快捷地绘制出精美...

问题 报错Error in plot.new() : figure margins too large怎么解决

跟老师的直播课上的做柱状图的视频中，转化完矩阵后，运行barplot时， 出现报错，不知道怎么解决。 > barplot(t,col=mycol[1:nrow(t)],ylab="Relative abundance ") Error in plot.new() : figure margins too large

文章 eQTplot包绘图报错解决：“Caused by error in `db_collect()`”

使用eQTplot包绘图时报错“Caused by error in `db_collect()`”： 大概查询了一下，应该是eQTplot需要调用dbplyr去查询ensemble数据库，dbplyr的功能描述——远程数据库查询： 最新版本的dbplyr和BiocFileCache存在不兼容的问题，对此官方...

问题 重测序circos运行失败

... CIRCOS ERROR ***       cwd: /work/my_reseq/8.circos       command: /share/work/biosoft/circos/latest/bin//circos -conf       /work/my_reseq/8.circos/circos.conf -outputdir /work/my_reseq/8.circos       -outputfile circos   CONFIGURATION FILE ERROR   Could not parse value...

问题 cellranger count 报错：job failed in stage code怎么解决

问题 请问，有些文献中，给出的KME值，在WGCNA教程中，生成的"10_GS_and_MM.xls"文件中，哪个和它是对应的？比如下图所示。文献链接如下：https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5429000/

文章 基因家族分析课程更新2.0

...参考文献： Zhang, Z., Xiao, J., Wu, J., Zhang, H., Liu, G., Wang, X. and Dai, L. (2012) ParaAT: A parallel tool for constructing multiple protein-coding DNA alignments, Biochem Biophys Res Commun, 419(4):779-7 trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyse...