用户 Man in the Mirror

用户 IN0vation

问题 kneaddata做质控的时候，服务器卡死了，用trimmomatic+bowtie2分开跑是可以的吧

... "$datafile"/{}_1.fq.gz "$datafile"/{}_2.fq.gz \   "$workdir"/1.host_del_and_qc/kneaddata/{}_forward_paired.fq.gz "$workdir"/1.host_del_and_qc/kneaddata/{}_forward_unpaired.fq.gz \   "$workdir"/1.host_del_and_qc/kneaddata/{}_reverse_paired.fq.gz "$workdir"/1.host_del_and_qc/kneaddata/{}_reverse_...

问题 samtools faidx: different length in sequence

您好! 您在2020年8月用samtools获取染色体长度时有一个different line length in sequence contig1的报错，这个问题解决了嘛？ 我最近也遇到了相同的问题

问题 dadi

...by the 14 tested models (Figs. 2A, S1), with a log‐likelihood of 141.756 and an AIC of 1354.21 (Table 1). Parameter estimates inferred that the divergence time of LO and LO was 0.61 Ma (95% CIs: 0.58–0.65), and the two diverged species had a second contact at 7109 years ago (95% CIs: 6633–7585...

文章 快速解读：3-5分的蛋白质组学文章思路

iTRAQ (Isobaric tag for relative and absolute quantitation，iTRAQ）和TMT（Tandem Mass Tag）是近年来应用最广泛的差异蛋白质组学技术，它们均采用体外标记的方法，利用同位素试剂标记蛋白质酶解后产生的多肽，对两个或多个样本，在全蛋白质...

问题 扩增子分析：qiime 聚类生成OTU-denovo方法分析报错

...lib/python2.7/site-packages/qiime/workflow/util.py", line 122, in call_commands_serially     raise WorkflowError(msg) qiime.workflow.util.WorkflowError: *** ERROR RAISED DURING STEP: Assign taxonomy Command run was:  assign_taxonomy.py -o pick_de_novo_otus/uclust_assigned_taxonomy -i pick_...

问题 请问大佬，做转录组分析时，fpkm_and_plot.R分析数据时，出现错误Error: Aesthetics must be either length 1 or the same as the data (100276): x，这个怎么解决呀？

文章 monocle2 monocle2 igraph 报错

monocle2 igraph 报错 Error:! `nei()` was deprecated in igraph 2.1.0 and is now defunct.ℹ Please use `.nei()` instead. --- Backtrace:▆ 1. ├─monocle::orderCells(cds) 2. │ └─monocle:::project2MST(cds, project_point_to_line_segment) 3. │ ├─V(dp_mst)[suppressWarnings(nei(c...

文章 wgd syn 报错 KeyError: 6.0

...ack (most recent call last):   File "/share/work/biosoft/python/Python3/bin/wgd", line 33, in <module>     sys.exit(load_entry_point('wgd==1.2', 'console_scripts', 'wgd')())   File "/share/work/biosoft/python/Python-v3.9.16/lib/python3.9/site-packages/click/core.py", line 1130, in __cal...

文章 VirtualBox 中练习安装linux系统centos7

...Universityhttp://mirrors.tuna.tsinghua.edu.cn/centos/University of Science and Tech of Chinahttp://mirrors.ustc.edu.cn/centos/Zhejiang Universityhttp://mirrors.zju.edu.cn/centos/ 清华的镜像地址：https://mirrors.tuna.tsinghua.edu.cn/centos/7.9.2009/isos/x86_64/  版本说明 CentOS-7-x8...

问题 单细胞测序数据差异基因KEGG富集分析

...ENE"     "UNIPROT"     'select()' returned 1:1 mapping between keys and columns Reading KEGG annotation online: Reading KEGG annotation online: --> No gene can be mapped.... --> Expected input gene ID:  --> return NULL... NULL Error in (function (classes, fdef, mtable)  ...

问题 请问request returned Internal Server Error for API route and version http://%2F%2F.%2Fpipe%2Fdocker_engine/v1.24/containers... check if the server supports the requested API version 这个问题该怎么解决

...containers中出现 request returned Internal Server Error for API route and version http://%2F%2F.%2Fpipe%2Fdocker_engine/v1.24/containers/025fef2a3e783d8c473c644d58e2ffd31be5f157743e797b0649966a18031dfc/json, check if the server supports the requested API version

问题 boxplot画图提示“Error in oldClass(stats)

问题 WGCNA分析过程中做样品聚类树与形状热图时遇到问题

做性状热图的那一步时，出现了： Error in numbers2colors(datTraits, signed = FALSE) :    'lim' must be finite. In addition: Warning messages: 1: In FUN(newX[, i], ...) : no non-missing arguments to min; returning Inf 2: In FUN(newX[, i], ...) : no non-missing arguments to min; re...