基因组注释:10.PASA_update,根据fa和gff文件生成pep或者cdna文件,一直显示:
No such file or directory at /share/work/biosofTransDecoder/TransDecoder-v5.5.0/util/../PerlLib/GFF3_utils2.pm line 37, <$filehandle> line 112.
... files are not indexed.
Please index all input files:
samtools index SRR3274663.new.sorted.bam
Set the system property GATK_STACKTRACE_ON_USER_EXCEPTION (--java-options '-DGATK_STACKTRACE_ON_USER_EXCEPTION=true') to print the stack trace.
这是我的命令:gatk --java-options "-Xmx20g -Djava....