做有参转录组分析,在进行基因表达定量的时候,其中一个转录组出现如下错误,看不太懂,想请教一下老师问题如何解决

attachments-2019-12-ewj0iRJa5df831453e9d6.png19000000 SAM alignment record pairs processed.
19100000 SAM alignment record pairs processed.
19200000 SAM alignment record pairs processed.
19300000 SAM alignment record pairs processed.
19400000 SAM alignment record pairs processed.
19500000 SAM alignment record pairs processed.
19600000 SAM alignment record pairs processed.
19700000 SAM alignment record pairs processed.
Error occured when processing SAM input (record #42564640 in file /bioData/run_data/lixp/work/SZ_yangtao1/2.alignment/hisat2/yangtaozhitiao1.bam):
  Maximum alignment buffer size exceeded while pairing SAM alignments.
  [Exception type: ValueError, raised in __init__.py:671]




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1 个回答

omicsgene - 生物信息
擅长:重测序,遗传进化,转录组,GWAS

可能是内存,或者存储不足

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