一般，警告问题不大
做有参转录组分析，在进行基因表达定量的时候，出现如下警告，请问老师这是什么原因呀
23900000 SAM alignment record pairs processed.
Warning: Mate records missing for 270457 records; first such record: <SAM_Alignment object: Paired-end read 'A00224:87:H5WHYDSXX:1:2474:9118:3098' aligned to utg9_pilon_pilon_pilon:[3553256,3553424)/+>.
24000000 SAM alignment record pairs processed.
24100000 SAM alignment record pairs processed.
24200000 SAM alignment record pairs processed.
Warning: Mate pairing was ambiguous for 113851 records; mate key for first such record: ('A00224:87:H5WHYDSXX:1:2228:10402:29089', 'first', 'utg2_pilon_pilon_pilon', 72835, 'utg2_pilon_pilon_pilon', 73837, 334).
24211516 SAM alignment pairs processed.
MSTRG.1 15
MSTRG.10 0
MSTRG.100 486
