老师您好！根据老师前几日给出的建议采用circos进行基因组共线性分析。link，text，genome文件按照老师课程中的格式准备。config1.txt 用的是老师给的参考资料中的文件，仅修改了输入文件。最后运行绘图命令时出现如下报错，请问老师这个是哪里出问题了呀？要怎么解决呢？谢谢老师~

  To see where Circos looks for the file, use

      circos -debug_flag io

  To see how configuration files work, create the example image, whose

  configuration and data are found in example/. From the Circos distribution

  directory,

      cd example

      ../bin/circos -conf ./circos.conf

  or use the 'run' script (UNIX only).

  Configuration files are described here

      http://circos.ca/tutorials/lessons/configuration/configuration_files/

  and the use of command-line flags, such as -conf, is described here

      http://circos.ca/tutorials/lessons/configuration/runtime_parameters/

  Windows users unfamiliar with Perl should read

      http://circos.ca/tutorials/lessons/configuration/unix_vs_windows/

  This error can also be produced if supporting configuration files, such as

  track defaults, cannot be read.

  If you are having trouble debugging this error, first read the best practices

  tutorial for helpful tips that address many common problems

      http://www.circos.ca/documentation/tutorials/reference/best_practices

  The debugging facility is helpful to figure out what's happening under the

  hood

      http://www.circos.ca/documentation/tutorials/configuration/debugging

  If you're still stumped, get support in the Circos Google Group.

      http://groups.google.com/group/circos-data-visualization

  Please include this error, all your configuration, data files and the version

  of Circos you're running (circos -v).Do not email me directly -- please use

  the group.

  Stack trace:

 at /biosoft/circos/circos-0.69-6/bin/../lib/Circos/Error.pm line 425.

Circos::Error::fatal_error('configuration', 'missing', '/home/manager/share1/cir/config1.txt') called at /biosoft/circos/circos-0.69-6/bin/../lib/Circos/Configuration.pm line 796

Circos::Configuration::loadconfiguration('/home/manager/share1/cir/config1.txt') called at /biosoft/circos/circos-0.69-6/bin/../lib/Circos.pm line 148

Circos::run('Circos', 'outputdir', '/home/manager/share1/cir/result', '_cwd', '/home/manager/share1/gene_duplication/cir', '_argv', '-conf /home/manager/share1/cir/config1.txt -outputdir /home/m...', 'outputfile', 'test1', ...) called at /biosoft/circos/circos-0.69-6/bin/circos line 536

chromosomes_units=1000000

#chromosomes_reverse=/chr[01]/

<ideogram>

    fill=yes

    label_font=default

    label_parallel=yes

    label_radius=dims(image,radius)-60p

    label_size=45

    radius=0.90r

    show_label=yes

    <spacing>

        default=0.005r

    </spacing>

    stroke_color=dgrey

    stroke_thickness=2p

    thickness=0.03r

</ideogram>

karyotype=/home/manager/share1/cir/chr.info

<links>

    bezier_radius=0r

    bezier_radius_purity=0.75

    color=black

    crest=0.5

    <link>

        bezier_radius=0r

        bezier_radius_purity=0.75

        color=set2-8-qual-1

        crest=0.5

        file=/home/manager/share1/cir/new_link.txt

        radius=0.88r

        <rules>

            <rule>

                color=red

                condition=var(intrachr)

            </rule>

            <rule>

                color=red

                condition=var(interchr)

            </rule>

        </rules>

        thickness=6

        z=20

    </link>

    <link>

        bezier_radius=0r

        bezier_radius_purity=0.75

        color=230,230,230,0.2

        crest=0.5

        ribbon=yes

        file=/home/manager/share1/cir/genome.txt

        radius=0.88r

        <rules>

            <rule>

                condition=var(intrachr)

            </rule>

            <rule>

                condition=var(interchr)

            </rule>

        </rules>

        thickness=1

        z=15

    </link>

    radius=0.40r

    thickness=1

</links>

<plots>

    <plot>

        color=set2-8-qual-2

        file=/home/manager/share1/cir/nwe_text.txt

        label_font=light

        link_color=black

        link_dims=0p,2p,5p,2p,2p

        link_thickness=2p

        r0=0.88r

        r1=0.99r

        rpadding=5p

        show_links=no

        type=text

    </plot>

    type=histogram

</plots>

show_tick_labels=yes

show_ticks=yes

spacing=10u

<ticks>

    color=black

    format=%d

    multiplier=1e-6

    radius=1r

    thickness=2p

    <tick>

        size=10p

        spacing=5u

    </tick>

    <tick>

        color=black

        format=%d

        label_offset=10p

        label_size=25p

        show_label=yes

        size=15p

        spacing=25u

        thickness=4p

    </tick>

</ticks>

<colors>

<<include etc/colors.conf>>

<<include etc/brewer.conf>>

#<<include etc/colors_fonts_patterns.conf>>

#<<include colors.ucsc.conf>>

#<<include colors.hsv.conf>>

</colors>

<fonts>

<<include etc/fonts.conf>>

</fonts>

<image>

<<include etc/image.conf>>

</image>

<<include etc/housekeeping.conf>>